Separation of R-form lipopolysaccharide and lipid A by CE-Fourier-transform ion cyclotron resonance MS
| Autor: | Göran Hübner, Buko Lindner |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Detection limit
Lipopolysaccharides Proteomics Chromatography Fourier Analysis Chemistry Clinical Biochemistry Analytical chemistry Electrophoresis Capillary Ion suppression in liquid chromatography–mass spectrometry Electrolyte Cyclotrons Mass spectrometry Biochemistry Fourier transform ion cyclotron resonance Mass Spectrometry Analytical Chemistry Lipid A Bacterial outer membrane Ion cyclotron resonance |
| Zdroj: | Electrophoresis. 30(10) |
| ISSN: | 1522-2683 |
| Popis: | Lipopolysaccharide (LPS) is the main component of the outer leaflet of the outer membrane of Gram-negative bacteria. Native isolates of LPS comprise a high inherent heterogeneity. This paper presents the first application of CE to separate and online mass analyze the different constituents of underivatized R-form LPS and lipid A with an FT ion cyclotron resonance mass spectrometer. A Beckman P/ACE MDQ and a home-made CE instrument were coupled to the mass spectrometer using a sheath liquid interface. An optimized CE electrolyte based on water, isopropyl alcohol, triethylamine and acetic acid at pH 8.9 allowed the separation of molecular species even of complex mixtures to a large extent with observed detection limits of single constituents of the samples in the low femtomol range. Comparison of CE-MS and MS measurements under equal conditions enabled the investigation of ion suppression effects in LPS samples. |
| Databáze: | OpenAIRE |
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