Royal jelly coordinately enhances hippocampal neuronal expression of somatostatin and neprilysin genes conferring neuronal protection against toxic soluble amyloid-β oligomers implicated in Alzheimer’s disease pathogenesis
Autor: | Kikuji Yamaguchi, Akira Fujii, Michiyo Takahashi, Tohru Yamakuni, Ichiro Kawahata, Huinan Xu, Wanying Han, Kiyoshi Murata, Yoshihisa Yamaguchi |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
endocrine system Royal jelly Medicine (miscellaneous) Biology Hippocampal formation 03 medical and health sciences 0302 clinical medicine fluids and secretions Transcription (biology) CRE-mediated transcription TX341-641 Receptor Neprilysin Nutrition and Dietetics CREB Nutrition. Foods and food supply fungi Antagonist Promoter Chromatin Cell biology 030104 developmental biology Somatostatin Hippocampal neurons 030217 neurology & neurosurgery hormones hormone substitutes and hormone antagonists Food Science |
Zdroj: | Journal of Functional Foods, Vol 51, Iss, Pp 28-38 (2018) |
ISSN: | 1756-4646 |
Popis: | Hippocampal somatostatin (SST)-neprilysin (NEP) system functions as a defense system against neurotoxic soluble amyloid-β oligomers associated with onset of Alzheimer’s disease. However, it remains unknown whether royal jelly (RJ) could impact the defense system. In this study using primary cultures of hippocampal neurons, including SST-positive and NEP-positive neuronal subsets, we evaluated the ability of DMSO-soluble fraction of RJ (DRJ) to enhance SST and NEP genes expression. The DRJ potently facilitated CRE-mediated transcription, and raised SST-immunoreactivity in an SST-positive neuronal subset, where elevated phospho-CREB immunoreactivity was simultaneously observed. Chromatin immunoprecipitation-qPCR assays uncovered that the DRJ mechanistically facilitated CREB-binding to a CRE present at the promoter region of SST. Moreover, in a distinct NEP-positive neuronal subset, the DRJ led to elevation of NEP immunoreactivity, which was abolished by cyclosomatostatin, a non-selective SST receptors antagonist. Collectively, these findings provide the first evidence that the RJ possesses potential to activate the hippocampal SST-NEP system. |
Databáze: | OpenAIRE |
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