Royal jelly coordinately enhances hippocampal neuronal expression of somatostatin and neprilysin genes conferring neuronal protection against toxic soluble amyloid-β oligomers implicated in Alzheimer’s disease pathogenesis

Autor: Kikuji Yamaguchi, Akira Fujii, Michiyo Takahashi, Tohru Yamakuni, Ichiro Kawahata, Huinan Xu, Wanying Han, Kiyoshi Murata, Yoshihisa Yamaguchi
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Functional Foods, Vol 51, Iss, Pp 28-38 (2018)
ISSN: 1756-4646
Popis: Hippocampal somatostatin (SST)-neprilysin (NEP) system functions as a defense system against neurotoxic soluble amyloid-β oligomers associated with onset of Alzheimer’s disease. However, it remains unknown whether royal jelly (RJ) could impact the defense system. In this study using primary cultures of hippocampal neurons, including SST-positive and NEP-positive neuronal subsets, we evaluated the ability of DMSO-soluble fraction of RJ (DRJ) to enhance SST and NEP genes expression. The DRJ potently facilitated CRE-mediated transcription, and raised SST-immunoreactivity in an SST-positive neuronal subset, where elevated phospho-CREB immunoreactivity was simultaneously observed. Chromatin immunoprecipitation-qPCR assays uncovered that the DRJ mechanistically facilitated CREB-binding to a CRE present at the promoter region of SST. Moreover, in a distinct NEP-positive neuronal subset, the DRJ led to elevation of NEP immunoreactivity, which was abolished by cyclosomatostatin, a non-selective SST receptors antagonist. Collectively, these findings provide the first evidence that the RJ possesses potential to activate the hippocampal SST-NEP system.
Databáze: OpenAIRE
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