Fecal excretion of a nonenveloped DNA virus (TTV) associated with posttransfusion non-A-G hepatitis
| Autor: | Masato Ukita, Yuzo Miyakawa, Fumio Tsuda, Yoshihiro Akahane, Makoto Mayumi, Hiroaki Okamoto, Masako Fukuda |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Torque teno virus
Carcinoma Hepatocellular Genotype Hepatitis Viral Human Molecular Sequence Data Biology Polymerase Chain Reaction Virus law.invention Transfusion transmitted virus Feces law Virology Centrifugation Density Gradient medicine Humans Phylogeny Polymerase chain reaction Hepatitis Base Sequence Liver Neoplasms DNA Viruses Transfusion Reaction DNA virus Sequence Analysis DNA medicine.disease biology.organism_classification DNA Virus Infections Infectious Diseases DNA Viral Viral hepatitis |
| Zdroj: | Journal of Medical Virology. 56:128-132 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/(sici)1096-9071(199810)56:2<128::aid-jmv5>3.0.co;2-a |
| Popis: | Five patients with type B or C hepatocellular carcinoma were found to be infected with a nonenveloped DNA virus (TTV) associated with posttransfusion hepatitis of non-A-G etiology. Paired feces and serum samples from these patients were tested for TTV DNA by polymerase chain reaction with seminested primers and their sequences were compared. TTV DNA was detected in sera from all of the patients, while it was detected in feces from three patients, including two with high viral titers in serum. When feces and serum from one patient were subjected to floatation ultracentrifugation in CsCl, TTV in feces banded at a peak density of 1.35 g/cm3 and that in serum at 1.31-1.32 g/cm3. TTV isolates in three pairs of feces and serum had the identical sequence of 222 base pairs. The excretion of TTV into feces indicates that TTV would be transmitted not only parenterally but also nonparenterally by a fecal-oral route. |
| Databáze: | OpenAIRE |
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