Massively parallel sequencing of forensically relevant single nucleotide polymorphisms using TruSeq™ forensic amplicon
| Autor: | Yonmee Han, David H. Warshauer, Kathryn M. Stephens, Tom Richardson, Paulina Walichiewicz, Bruce Budowle, Jonathan L. King, Cydne Holt, Carey Davis, Anne Jager |
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| Rok vydání: | 2014 |
| Předmět: |
Whole genome sequencing
Genetics Genetic Markers Heterozygote Massive parallel sequencing Sequence analysis High-Throughput Nucleotide Sequencing Single-nucleotide polymorphism Sequence Analysis DNA Biology Amplicon Real-Time Polymerase Chain Reaction Polymorphism Single Nucleotide Pathology and Forensic Medicine Genetic marker Genotype Humans Exome sequencing DNA Primers |
| Zdroj: | International journal of legal medicine. 129(1) |
| ISSN: | 1437-1596 |
| Popis: | The TruSeq™ Forensic Amplicon library preparation protocol, originally designed to attach sequencing adapters to chromatin-bound DNA for chromatin immunoprecipitation sequencing (TruSeq™ ChIP-Seq), was used here to attach adapters directly to amplicons containing markers of forensic interest. In this study, the TruSeq™ Forensic Amplicon library preparation protocol was used to detect 160 single nucleotide polymorphisms (SNPs), including human identification SNPs (iSNPs), ancestry, and phenotypic SNPs (apSNPs) in 12 reference samples. Results were compared with those generated by a second laboratory using the same technique, as well as to those generated by whole genome sequencing (WGS). The genotype calls made using the TruSeq™ Forensic Amplicon library preparation protocol were highly concordant. The protocol described herein represents an effective and relatively sensitive means of preparing amplified nuclear DNA for massively parallel sequencing (MPS). |
| Databáze: | OpenAIRE |
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