Prolonged intervals during Mycobacterium tuberculosis subunit vaccine boosting contributes to eliciting immunity mediated by central memory-like T cells
| Autor: | Yanping Luo, Liang Peng, Chunxiang Bai, Lina Hu, Bingdong Zhu, Xun Liu, He Juanjuan, Hongxia Niu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Tuberculosis Vaccination schedule medicine.medical_treatment T cell Immunology Microbiology 03 medical and health sciences 0302 clinical medicine Immune system T-Lymphocyte Subsets Immunity Animals Medicine Tuberculosis Vaccines Cells Cultured Immunization Schedule Antigens Bacterial Immunity Cellular business.industry ELISPOT Mycobacterium tuberculosis medicine.disease Mycobacterium bovis Mice Inbred C57BL Vaccination 030104 developmental biology Infectious Diseases Cytokine medicine.anatomical_structure Vaccines Subunit Cytokines Female business Immunologic Memory 030215 immunology |
| Zdroj: | Tuberculosis. 110:104-111 |
| ISSN: | 1472-9792 |
| DOI: | 10.1016/j.tube.2018.04.006 |
| Popis: | It is believed that central memory T cells (TCM) provide long-term protection against tuberculosis (TB). However, the effects of TB subunit vaccine immunization schedule, especially the vaccination intervals, on T cell immune memory is still unclear. In this study, mice were immunized with fusion protein ESAT6-Ag85B-MPT64 (190-198)-Mtb8.4-Rv2626c (LT70) based subunit vaccine three times according to the following schedules: ① 0, 3rd and 6th week respectively (0-3-6w), ② 0, 4th and 12th week (0-4-12w), and ③ 0, 4th and 24th week (0-4-24w). We found that both schedules of 0-4-12w and 0-4-24w induced higher level of antigen specific IL-2, IFN-γ and TNF-α than 0-3-6w immunization. Among them, 0-4-12w induced the highest level of IL-2, which is a key cytokine mainly produced by TCM. Moreover, by cultured IFN-γ ELISPOT and cell proliferation assay etc., we found that the vaccination schedule of 0-4-12w elicited higher numbers of TCM like cells, stronger TCM - mediated immune responses and higher protective efficacy against M. bovis BCG challenge than 0-3-6w did. It suggests that prolonging the vaccination interval of TB subunit vaccine to some extent contributes to inducing more abundant TCM like cells and providing stronger immune protection against mycobacteria infection. |
| Databáze: | OpenAIRE |
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