Endothelial Cell Processing and Alternatively Spliced Transcripts of Factor VIII: Potential Implications for Coagulation Cascades and Pulmonary Hypertension
| Autor: | Michael Laffan, Claire L. Shovlin, Richard A. Manning, Graeme M. Birdsey, Fatima S Govani, Gillian Angus, IG Mollet, Francesco Mauri, Grace N Okoli, Kay Elderfield |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Gene isoform
CD31 congenital hereditary and neonatal diseases and abnormalities Transcription Genetic Hypertension Pulmonary Hematology/Coagulation Disorders lcsh:Medicine Enzyme-Linked Immunosorbent Assay Biology Pulmonary Artery Flow cytometry Von Willebrand factor hemic and lymphatic diseases von Willebrand Factor medicine Weibel–Palade body Humans Protein Isoforms lcsh:Science Blood Coagulation Lung Cells Cultured Cardiovascular Disorders/Pulmonary Vascular Diseases Multidisciplinary Factor VIII Microscopy Confocal medicine.diagnostic_test Base Sequence Reverse Transcriptase Polymerase Chain Reaction lcsh:R Endothelial Cells Exons Molecular Biology/Transcription Initiation and Activation medicine.disease Flow Cytometry Molecular biology Pulmonary hypertension Immunohistochemistry Endothelial stem cell Alternative Splicing medicine.anatomical_structure biology.protein lcsh:Q Transcription Initiation Site Research Article Signal Transduction |
| Zdroj: | PLoS ONE e9154 PLoS ONE, Vol 5, Iss 2, p e9154 (2010) |
| ISSN: | 1932-6203 |
| Popis: | Background Coagulation factor VIII (FVIII) deficiency leads to haemophilia A. Conversely, elevated plasma levels are a strong predictor of recurrent venous thromboemboli and pulmonary hypertension phenotypes in which in situ thromboses are implicated. Extrahepatic sources of plasma FVIII are implicated, but have remained elusive. Methodology/Principal Findings Immunohistochemistry of normal human lung tissue, and confocal microscopy, flow cytometry, and ELISA quantification of conditioned media from normal primary endothelial cells were used to examine endothelial expression of FVIII and coexpression with von Willebrand Factor (vWF), which protects secreted FVIII heavy chain from rapid proteloysis. FVIII transcripts predicted from database mining were identified by rt-PCR and sequencing. FVIII mAb-reactive material was demonstrated in CD31+ endothelial cells in normal human lung tissue, and in primary pulmonary artery, pulmonary microvascular, and dermal microvascular endothelial cells. In pulmonary endothelial cells, this protein occasionally colocalized with vWF, centered on Weibel Palade bodies. Pulmonary artery and pulmonary microvascular endothelial cells secreted low levels of FVIII and vWF to conditioned media, and demonstrated cell surface expression of FVIII and vWF Ab–reacting proteins compared to an isotype control. Four endothelial splice isoforms were identified. Two utilize transcription start sites in alternate 5′ exons within the int22h-1 repeat responsible for intron 22 inversions in 40% of severe haemophiliacs. A reciprocal relationship between the presence of short isoforms and full-length FVIII transcript suggested potential splice-switching mechanisms. Conclusions/Significance The pulmonary endothelium is confirmed as a site of FVIII secretion, with evidence of synthesis, cell surface expression, and coexpression with vWF. There is complex alternate transcription initiation from the FVIII gene. These findings provide a framework for future research on the regulation and perturbation of FVIII synthesis, and of potential relevance to haemophilia, thromboses, and pulmonary hypertensive states. |
| Databáze: | OpenAIRE |
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