Variability of HHV8 LNA-1 Immunohistochemical Staining Across the 3 Histologic Stages of HIV-Associated Mucocutaneous Kaposi Sarcoma: Is There a Relationship to Patients' CD4 Counts?
| Autor: | Reena D. Mohanlal, Sugeshnee Pather |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Skin Neoplasms Mucocutaneous zone Human immunodeficiency virus (HIV) Dermatology medicine.disease_cause Pathology and Forensic Medicine Antigen medicine Humans Antigens Viral Sarcoma Kaposi Retrospective Studies AIDS-Related Opportunistic Infections business.industry Human herpes virus virus diseases Nuclear Proteins Retrospective cohort study General Medicine Herpesviridae Infections medicine.disease Phosphoproteins Immunohistochemistry CD4 Lymphocyte Count Cross-Sectional Studies Herpesvirus 8 Human Sarcoma business |
| Zdroj: | The American Journal of dermatopathology. 37(7) |
| ISSN: | 1533-0311 |
| Popis: | The histologic diagnosis of Kaposi sarcoma (KS) can be confirmed with human herpes virus 8 (HHV8) latency-associated nuclear antigen (LNA)-1 immunohistochemistry, which may show variability in distribution and intensity. This retrospective study was aimed at addressing the factors that may contribute to this variability. All cases of mucocutaneous KS diagnosed in a 5-year period at the histopathology department at a tertiary hospital in South Africa with available patients' CD4 counts and HHV8 LNA-1 immunohistochemically stained slides were reviewed, and the biopsy stages of KS (patch/plaque/nodular), CD4 counts, immunohistochemistry staining method (manual vs. automated), and distribution (diffuse/focal) and intensity (strong/weak) of HHV8 LNA-1 staining were recorded. A total of 127 cases were reviewed. No relationship was demonstrated between the median CD4 count and the histologic stages of KS (P = 0.701) or the intensity and distribution of HHV8 immunohistochemical staining using either staining method. Multivariate analysis showed that method of immunohistochemical staining was a significant predictor of distribution (P = 0.006) and intensity (P = 0.044) of staining, and that stage was a significant predictor of distribution of staining (P = 0.033). |
| Databáze: | OpenAIRE |
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