Isolation and characterization of alphoid DNA sequences specific for the pericentric regions of chromosomes 4, 5, 9, and 19
| Autor: | H.H. Ropers, Jan Schepens, M. Coerwinkel-Driessen, D. Schonk, Bé Wieringa, T. Hulsebos, I. van Dalen |
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| Rok vydání: | 1988 |
| Předmět: |
Genetics
Base Sequence Satellite DNA Centromere Molecular Sequence Data Chromosome Nucleic Acid Hybridization DNA Restriction Enzymes Biology DNA Satellite Genome DNA sequencing Chromosome 19 Sequence Homology Nucleic Acid Cosmid Humans Chromosomes Human 4-5 Cloning Molecular Repeated sequence Molecular probe Chromosomes Human Pair 9 Molecular Biology Chromosomes Human Pair 19 Genetics (clinical) |
| Zdroj: | Cytogenetics and cell genetics. 47(3) |
| ISSN: | 0301-0171 |
| Popis: | We have cloned and characterized two distinct types of alphoid DNA elements. Probe pG-Xbal 1/340 was obtained by random cloning of human satellite DNA and contains two basic units with overall 88% homology to the 171 -bp consensus alphoid sequence. pG-Xball/340-like elements are represented about 2,000-4,000 times in the haploid genome and, by in situ hybridization, are found exclusively at the primary constrictions of chromosomes 4 and 9. Probe pG-A16 was cloned from a chromosome 19-specific cosmid library and represents a 2.25-kb higher-order DNA element which is present at roughly 75-150 copies per haploid genome and which hybridizes to the centromeres of chromosomes 5 and 19. Using the pG-A16 probe, further genetic and physical dissection of the central area of chromosome 19 can be envisaged. |
| Databáze: | OpenAIRE |
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