THYROID STIMULATION BY (FAB)2AND FAB FRAGMENTS OF TSH RECEPTOR ANTIBODY
| Autor: | J. Ginsberg, F. A. Hashim, B. Rees Smith, E. Tunn, F. M. Creagh, Arthur B Parkes |
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| Rok vydání: | 1985 |
| Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism Radioimmunoassay Thyroid Gland Receptors Cell Surface Stimulation Antibodies Immunoglobulin Fab Fragments chemistry.chemical_compound Endocrinology Fab Fragments Internal medicine Cyclic AMP medicine Humans Receptor Cells Cultured Autoimmune disease biology Thyroid Receptors Thyrotropin medicine.disease Graves Disease Peptide Fragments Stimulation Chemical Papain medicine.anatomical_structure chemistry biology.protein Antibody |
| Zdroj: | Clinical Endocrinology. 23:175-183 |
| ISSN: | 1365-2265 0300-0664 |
| DOI: | 10.1111/j.1365-2265.1985.tb00213.x |
| Popis: | The TSH receptor binding and thyroid stimulating properties of (Fab)2 and Fab fragments of Graves' IgG have been investigated. (Fab)2 fragments were prepared by pepsin digestion of IgG and Fab fragments by reduction of (Fab)2 or papain digestion of IgG. (Fab)2 and Fab were effective in inhibiting TSH binding to its receptor with all five patients' sera studied and both preparations stimulated cyclic AMP release from isolated thyroid cells. However Fab fragments were less active thyroid stimulators than their parent (Fab)2 in all five cases. These studies indicate that antibody divalency is not essential for thyroid stimulation by TSH receptor antibodies. |
| Databáze: | OpenAIRE |
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