A* protein of bacteriophage φX174 carries an oligonucleotide which it can transfer to the 3−OH of a DNA chain
| Autor: | H.A.A.M. van Teeffelen, J. Zandberg, A.D.M. van Mansfeld, H.S. Jansz, G. H. Veeneman, J. H. Van Boom, P.D. Baas |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Oligonucleotides
Biophysics Biology Cleavage (embryo) Biochemistry Synthetic oligonucleotide Bacteriophage Bacteriophage φX174 Viral Proteins Endonuclease DDB1 Structural Biology Genetics A-DNA Molecular Biology Gene A* protein Base Sequence Oligonucleotide DNA Cell Biology biology.organism_classification Molecular biology biology.protein DNA-ligating activity Electrophoresis Polyacrylamide Gel Protein G DNA—protein complex Bacteriophage phi X 174 |
| Zdroj: | FEBS Letters. (1):103-108 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(82)81313-6 |
| Popis: | The bacteriophage φX174 gene A encodes two proteins: gene A protein and A* protein. Purified A* protein acts as a single-stranded, DNA-specific endonuclease which remains covalently attached to the 5′-end of the cleavage site. Incubation of A* protein with the synthetic heptamer CAACTTG or with oligonucleotides which yield this heptamer after cleavage with the A* protein yields oligonucleotides with the sequences CAACTTGAG, CAACTTGAGG and CAACTTGAGGA. This indicates that A* protein carries an oligonucleotide with the sequence -AG, AGG or -AGGA. The oligonucleotide can be transferred to the 3′-end of the heptamer CAACTTG. This suggests that A* protein reacts with a specific DNA sequence in the infected cell. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|