Effects of tanshinone IIA on fibrosis in a rat model of cirrhosis through heme oxygenase-1, inflammation, oxidative stress and apoptosis
| Autor: | Shun Zhang, Dam‑Dan Huang, Xiao‑Rong Hu, Ming Shu, Zuo‑An Hung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Cancer Research Pharmacology medicine.disease_cause Biochemistry Salvia miltiorrhiza p38 Mitogen-Activated Protein Kinases 0302 clinical medicine Fibrosis chemistry.chemical_classification biology Glutathione peroxidase Anti-Inflammatory Agents Non-Steroidal apoptosis Articles tanshinone IIA Oncology 030220 oncology & carcinogenesis Molecular Medicine Cytokines Inflammation Mediators heme oxygenase 1 Superoxide dismutase 03 medical and health sciences Genetics medicine Animals cirrhotic Protein kinase A Molecular Biology Protein kinase B Inflammation business.industry medicine.disease Rats Heme oxygenase Disease Models Animal Oxidative Stress 030104 developmental biology chemistry Immunology Abietanes biology.protein business Proto-Oncogene Proteins c-akt Oxidative stress Biomarkers Heme Oxygenase-1 |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Tanshinone IIA is extracted from the root of Salvia miltiorrhiza and used in traditional Chinese medicine for its anti-inflammatory activity and antioxidant effects. The aim of the present study was to investigate the potential protective effects of tanshinone IIA against fibrosis in a rat model of cirrhosis and to elucidate the underlying mechanisms. Male Sprague Dawley rats were used as the model of cirrhosis in the present study. In the cirrhotic rats, the extent of fibrosis, levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), heme oxygenase‑1 (HO‑1) protein expression, serum levels of nuclear factor (NF)‑κB, tumor necrosis factor‑α (TNF‑α), interleukin (IL)‑1β and IL‑6, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH‑PX), and the protein expression levels of phosphorylated‑p38 mitogen‑activated protein kinase (MAPK) were all significantly increased. However, the serum malondialdehyde (MDA) activity and protein kinase B (Akt) protein expression were suppressed in cirrhotic rats compared with the sham (control) group. Compared with the cirrhotic group, administration of tanshinone IIA reduced the extent of fibrosis, levels of ALT and AST, HO‑1 protein expression, serum NF‑κB, TNF‑α, IL‑1β and IL‑6 levels, and the activity of SOD, CAT and GSH‑PX. Furthermore, administration of tanshinone IIA significantly increased the inhibition of the serum MDA activity and the Akt protein expression in cirrhotic rats compared with those in the cirrhotic group. The protective effect of tanshinone IIA suppresses fibrosis in a rat model of cirrhosis, and reduces inflammation and oxidative stress, via the HO‑1, Akt and p38 MAPK signaling pathway. |
| Databáze: | OpenAIRE |
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