Upregulation of gut cathepsin L during Eimeria tenella infection

Autor: Gilles Lalmanach, Geneviève Fort, Pauline Gaboriaud, Lise Vanderlynden, Edouard Guitton, Catherine Schouler, Pauline Vettori, Fabien Lecaille, Françoise I. Bussière, Aurélie Fessard, Anne-Christine Lalmanach, Ahlame Saidi, Rodrigo Guabiraba, Fabrice Laurent
Přispěvatelé: Centre d'Etude des Pathologies Respiratoires (CEPR), UMR 1100. Equipe 2 'Mécanismes Protéolytiques dans l'Inflammation' (CEPR. Equipe 2), Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme d'Infectiologie Expérimentale (PFIE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Région Centre-Val de Loire, France (APR-IA 2017 17 'INTEGRITY' - N° 201700119982, INSERM (institutional funding), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Rok vydání: 2021
Předmět:
Zdroj: Research in Veterinary Science
Research in Veterinary Science, Elsevier, 2021, 140, pp.109-116. ⟨10.1016/j.rvsc.2021.08.013⟩
ISSN: 1532-2661
0034-5288
DOI: 10.1016/j.rvsc.2021.08.013⟩
Popis: International audience; Coccidiosis is a disease caused by Eimeria, which represents the first parasitic disease in poultry farming. Among them, E. tenella is a virulent species which specifically colonizes the caecum. The inflammatory response to infection is associated to numerous host proteases including cysteine cathepsins that can be deleterious for tissue and innate immunity integrity. Here, germ-free and conventional chickens were used as models to find out whether the microbiota could modify the intestinal expression of host cysteine cathepsins during coccidiosis. The basal caecal peptidase activity primarily relies on host proteases rather than proteases from the commensal flora. While mRNA levels of E. tenella cathepsins B and L remained unchanged in germ-free and conventional broilers, an overall increase in endopeptidase activity of cysteine cathepsins was found in E. tenella-infected caeca in both experimental models (P < 0.005). A significant decrease in avian cystatin C transcription was also observed in infected conventional, but not in infected germ-free broilers. Despite an unchanged mRNA level of avian cathepsin L (CatL), its protein expression raised following infection, in parallel with an increased transcription of antimicrobial β-defensins (AvBD1, AvBD2, AvBD4, AvBD6, and AvBD7). Taken together, data support that host CatL is post-translationally upregulated during E. tenella infection, and thus may be involved in the alteration of the gut proteolytic balance. Furthermore, CatL may participate to inflammation occurring during coccidiosis through its known ability to proteolytically inactivates up-regulated avian β-defensins that are key molecules of innate immunity.
Databáze: OpenAIRE
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