Application of RLEP Real-Time PCR for Detection of M. leprae DNA in Paraffin-Embedded Skin Biopsy Specimens for Diagnosis of Paucibacillary Leprosy
| Autor: | Fu Yue Tan, Rong De Yang, Lian Chao Yuan, Wen Yan, Yan Xing, Ying Zhang, Huan Ying Li |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
DNA Bacterial Male Pathology medicine.medical_specialty Adolescent Biopsy Real-Time Polymerase Chain Reaction Sensitivity and Specificity Skin Diseases law.invention Young Adult chemistry.chemical_compound law Virology medicine TaqMan Humans Child Mycobacterium leprae Polymerase chain reaction Aged Skin Paraffin Embedding biology medicine.diagnostic_test Articles Middle Aged biology.organism_classification medicine.disease Bacterial Load Interspersed Repetitive Sequences Infectious Diseases Real-time polymerase chain reaction chemistry Case-Control Studies Skin biopsy Female Parasitology Leprosy Paucibacillary Leprosy DNA |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 90:524-529 |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.13-0659 |
| Popis: | The TaqMan real-time polymerase chain reaction (PCR) assay was evaluated systematically with respect to the standard curve, linear range, and used for detecting Mycobacterium leprae DNA in paraffin-embedded skin biopsy specimens from 60 confirmed leprosy patients and three healthy individuals and 29 other dermatoses and bacterial DNA from 21 different species. The test was further evaluated with 51 paucibacillary (PB) patients. The results showed that the test had good sensitivity (8 fg) and good specificity with no cross-reactivity with 21 other bacterial species and the control specimens, except one with Xanthomatosis. The real-time PCR detection rate for the 51 PB specimens was 74.5% (38 of 51). We conclude that the real-time PCR test is a useful adjunct test for diagnosing early stage or PB leprosy cases. |
| Databáze: | OpenAIRE |
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