Existence of log-phase Escherichia coli persisters and lasting memory of a starvation pulse
| Autor: | Mikkel Skjoldan Svenningsen, Michael Sørensen, Namiko Mitarai, Sine Lo Svenningsen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
medicine.drug_class
Health Toxicology and Mutagenesis Antibiotics Population Plant Science Bacterial growth medicine.disease_cause Biochemistry Genetics and Molecular Biology (miscellaneous) Microbiology Exponential growth medicine TOLERANCE education Escherichia coli Starvation education.field_of_study Generation time Ecology Chemistry GENE Ciprofloxacin STRINGENT BACTERIAL PERSISTENCE GROWTH RNA medicine.symptom SYSTEM medicine.drug |
| Zdroj: | Svenningsen, M S, Svenningsen, S L, Sørensen, M A & Mitarai, N 2022, ' Existence of log-phase Escherichia coli persisters and lasting memory of a starvation pulse ', Life Science Alliance, vol. 5, no. 2, e202101076 . https://doi.org/10.26508/lsa.202101076 |
| Popis: | The vast majority of a bacterial population is killed when treated with a lethal concentration of antibiotics. The time scale of this killing is often comparable with the bacterial generation time before the addition of antibiotics. Yet, a small subpopulation typically survives for an extended period. However, the long-term killing dynamics of bacterial cells has not been fully quantified even in well-controlled laboratory conditions. We constructed a week-long killing assay and followed the survival fraction of Escherichia coli K12 exposed to a high concentration of ciprofloxacin. We found that long-term survivors were formed during exponential growth, with some cells surviving at least 7 d. The long-term dynamics contained at least three time scales, which greatly enhances predictions of the population survival time compared with the biphasic extrapolation from the short-term behavior. Furthermore, we observed a long memory effect of a brief starvation pulse, which was dependent on the (p)ppGpp synthase relA. Specifically, 1 h of carbon starvation before antibiotics exposure increased the surviving fraction by nearly 100-fold even after 4 d of ciprofloxacin treatment. |
| Databáze: | OpenAIRE |
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