Rapid determination of hydrophilic phenols in olive oil by vortex-assisted reversed-phase dispersive liquid-liquid microextraction and screen-printed carbon electrodes
| Autor: | Elena Fernández, Antonio Canals, Lorena Vidal |
|---|---|
| Přispěvatelé: | Universidad de Alicante. Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Alicante. Instituto Universitario de Materiales, Espectroscopía Atómica-Masas y Química Analítica en Condiciones Extremas |
| Rok vydání: | 2017 |
| Předmět: |
food.ingredient
Liquid Phase Microextraction Centrifugation 02 engineering and technology Screen-printed electrodes Hydrophilic phenols 01 natural sciences Analytical Chemistry Ferulic acid chemistry.chemical_compound food Caffeic Acids Phenols Oleuropein Olive oil samples Sunflower Oil Sample preparation Electrodes Olive Oil Reversed-phase dispersive liquid-liquid microextraction Chromatography Sunflower oil 010401 analytical chemistry Reproducibility of Results Electrochemical Techniques Phenylethyl Alcohol 021001 nanoscience & nanotechnology Carbon 0104 chemical sciences Hexane Tyrosol chemistry Calibration Hydroxytyrosol Química Analítica 0210 nano-technology Hydrophobic and Hydrophilic Interactions Oxidation-Reduction |
| Zdroj: | RUA. Repositorio Institucional de la Universidad de Alicante Universidad de Alicante (UA) |
| ISSN: | 1873-3573 |
| Popis: | A novel approach is presented to determine hydrophilic phenols in olive oil samples, employing vortex-assisted reversed-phase dispersive liquid-liquid microextraction (RP-DLLME) for sample preparation and screen-printed carbon electrodes for voltammetric analysis. The oxidation of oleuropein, hydroxytyrosol, caffeic acid, ferulic acid and tyrosol was investigated, being caffeic acid and tyrosol selected for quantification. A matrix-matching calibration using sunflower oil as analyte-free sample diluted with hexane was employed to compensate matrix effects. Samples were analyzed under optimized RP-DLLME conditions, i.e., extractant phase, 1 M HCl; extractant volume, 100 µL; extraction time, 2 min; centrifugation time, 10 min; centrifugation speed, 4000 rpm. The working range showed a good linearity between 0.075 and 2.5 mg L−1 (r = 0.998, N = 7) for caffeic acid, and between 0.075 and 3 mg L−1 (r = 0.999, N = 8) for tyrosol. The methodological limit of detection was empirically established at 0.022 mg L−1 for both analytes, which is significantly lower than average contents found in olive oil samples. The repeatability was evaluated at two different spiking levels (i.e., 0.5 mg L−1 and 2 mg L−1) and coefficients of variation ranged from 8% to 11% (n = 5). The applicability of the proposed method was tested in olive oil samples of different quality (i.e., refined olive oil, virgin olive oil and extra virgin olive oil). Relative recoveries varied between 83% and 108% showing negligible matrix effects. Finally, fifteen samples were analyzed by the proposed method and a high correlation with the traditional Folin-Ciocalteu spectrophotometric method was obtained. Thereafter, the concentrations of the fifteen oil samples were employed as input variables in linear discriminant analysis in order to distinguish between olive oils of different quality. The authors would like to thank Spanish Ministry of Science and Innovation (Project no. CTQ2011-23968), Spanish Ministry of Economy, Industry and Competitiveness (Project no. CTQ2016-79991-R, AEI/FEDER, UE) and Generalitat Valenciana (Spain) (Projects n. GVA/2014/096 and PROMETEO/2013/038) for the financial support. E. Fernández also thanks Spanish Ministry of Education for her FPU grant (FPU13/03125). |
| Databáze: | OpenAIRE |
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