Capping of ricin-binding sites does not influence phagocytosis in human polymorphonuclear leukocytes
| Autor: | Marco Baggiolini, Martin E. Feigenson |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
endocrine system
Binding Sites Chemistry Phagocytosis Cell Membrane hemic and immune systems Hematology General Medicine Ricin Microbiology carbohydrates (lipids) enzymes and coenzymes (carbohydrates) chemistry.chemical_compound Biochemistry Leukocytes Humans Binding site circulatory and respiratory physiology |
| Zdroj: | Acta haematologica. 59(3) |
| ISSN: | 0001-5792 |
| Popis: | Human polymorphonuclear leukocytes (PMN'S) were incubated at 0 degrees C with ferritin-conjugated ricin and warmed to 37 degrees C to induce capping of the ricin-binding sites. The PMNs were then allowed to phagocytose yeast or Staphylococcus epidermidis for 15 min, and processed for electron microscopy. Phagocytic uptake, granule fusion, and the fate of lectin-bound membrane were quantified by morphometry. Ricin-capped PMNs phagocytosed as extensively as untreated PMNs. Particles were ingested almost exclusively with a lectin-free portion of the plasmalemma. Fusion of granules with phagocytic vacuoles was not affected by ricin-induced capping. This indicates that ricin-binding sites are not involved in particle recognition and uptake. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|