A novel mcl PHA-producing bacterium, Pseudomonas guezennei sp. nov., isolated from a 'kopara' mat located in Rangiroa, an atoll of French Polynesia

Autor: Joelle Cozien, Sebastien Colin, Jean Guezennec, Karine Alain, Bernard Costa, Gérard Raguénès, Christelle Simon-Colin
Přispěvatelé: Centre de Brest, BIOMAR/BMM, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire d'océanographie biologique de Banyuls (LOBB), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Biolib, Biotechnologie et Molécules Marines (BMM), Biotechnologies et Ressources Marines (BRM), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
Rok vydání: 2007
Předmět:
MESH: Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics
Phylogenetics and taxonomy
Applied Microbiology and Biotechnology
MESH: Genotype
Microbial mat
polyhydroxyalkanaote
0303 health sciences
PHAs
Polyhydroxyalkanoates
Pseudomonas
Temperature
MESH: Polynesia
General Medicine
Hydrogen-Ion Concentration
MESH: Temperature
MESH: Glucose
Phenotype
kopara
Pseudomonadales
MESH: Microscopy
Electron
Transmission
Water Microbiology
Biotechnology
Mesophile
Pseudomonadaceae
Genotype
Heterotroph
Biology
MESH: Phenotype
Kopara
Gas Chromatography-Mass Spectrometry
Polynesia
Microbiology
03 medical and health sciences
Microscopy
Electron
Transmission
MESH: Polyhydroxyalkanoates
Seawater
030304 developmental biology
Polyhydroxyalkanaote
030306 microbiology
MESH: Magnetic Resonance Spectroscopy
MESH: Seawater
MESH: Pseudomonas
biology.organism_classification
microbial mat
MESH: Gas Chromatography-Mass Spectrometry
MESH: Water Microbiology
Glucose
Bacteria
Zdroj: Journal of Applied Microbiology
Journal of Applied Microbiology, Wiley, 2008, 104 (2), pp.581-586. ⟨10.1111/j.1365-2672.2007.03568.x⟩
Journal of Applied Microbiology, 2008, 104 (2), pp.581-586. ⟨10.1111/j.1365-2672.2007.03568.x⟩
Journal of Applied Microbiology (1364-5072) (Blackwell science), 2008-02, Vol. 104, N. 2, P. 581-586
ISSN: 1365-2672
1364-5072
DOI: 10.1111/j.1365-2672.2007.03568.x⟩
Popis: The definitive version is available at ww3.interscience.wiley.com; International audience; Aims: The aim of the present study was to describe an aerobic, mesophilic and heterotrophic bacterium, designated RA26, able to produce a medium-chain-length polyhydroxyalkanoate (PHA). It was isolated from a French Polynesian bacterial mat located in the atoll of Rangiroa. Methods and Results: This micro-organism, on the basis of the phenotypical features and genotypic investigations can be clearly assigned to the Pseudomonas genus and the name of Pseudomonas guezennei is proposed. Optimal growth occurs between 33 and 37°C, at a pH between 6·4 and 7·1 and at ionic strength of 15 g l−1 of sea salts. The G+C content of DNA is 63·2%. Under laboratory conditions, this bacterium produced a novel, medium-chain-length PHA, mainly composed of 3-hydroxydecanaote (64 mol.%) and 3-hydroxyoctanoate (24 mol.%) (GC-MS, NMR) from a single nonrelated carbon substrate, i.e. glucose. Conclusions: The bacterium P. guezennei produces a novel PHA mcl with elastomeric properties. Significance and Impact of the Study: PHAs share physical and material properties that recommend them for application in various areas, and are considered as an alternative to nonbiodegradable plastics produced from fossil oils. In this study, we describe a new bacteria with the capability to synthesize a novel PHA with promising biotechnological applications.
Databáze: OpenAIRE
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