Enhanced plasma persistence of therapeutic enzymes by coupling to soluble dextran
| Autor: | D A Rutter, Tony Atkinson, R F Sherwood, C N Wiblin, J K Baird, D C Ellwood |
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| Rok vydání: | 1977 |
| Předmět: |
History
Arginine Biological Availability Carboxypeptidases Education Mice chemistry.chemical_compound Pharmaceutic Aids Animals chemistry.chemical_classification Arginase biology Chemistry Dextrans Neoplasms Experimental Carbohydrate Enzyme assay Computer Science Applications Carboxypeptidase G Enzyme Dextran Biochemistry biology.protein Female Research Article Conjugate |
| Zdroj: | Biochemical Journal. 164:461-464 |
| ISSN: | 0306-3283 |
| DOI: | 10.1042/bj1640461 |
| Popis: | Conjugation of carboxypeptidase G and arginase, two enzymes of therapeutic interest, to a soluble dextran significantly enhanced plasma persistence in normal and tumour-bearing mice. A prolonged decrease in arginine concentrations in plasma of tumour-bearing mice was demonstrated by using the dextran-linked arginase. Gel filtration of dextran-enzyme conjugate showed that enzyme activity co-chromatographed as a single peak with carbohydrate, and enzyme was shown to be covalently linked to the dextran. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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