Methyl thiophanate-induced toxicity in liver and kidney of adult rats: a biochemical, molecular and histopathological approach

Autor: Y. Ben Ali, Ons Boudawara, K. M. Zeghal, Imen Jaballi, H Ben Saad, Amal Feki, Ahmed Hakim, I. Ben Amara, Christian Magné
Rok vydání: 2017
Předmět:
Male
Gene Expression
Ascorbic Acid
DNA Fragmentation
010501 environmental sciences
Kidney
medicine.disease_cause
01 natural sciences
Lethal Dose 50
Protein Carbonylation
Superoxide dismutase
chemistry.chemical_compound
Malondialdehyde
Toxicity Tests
Acute
medicine
Animals
Rats
Wistar
0105 earth and related environmental sciences
chemistry.chemical_classification
Glutathione Peroxidase
Reactive oxygen species
Dose-Response Relationship
Drug
biology
Reverse Transcriptase Polymerase Chain Reaction
Superoxide Dismutase
Glutathione peroxidase
010401 analytical chemistry
Hydrogen Peroxide
General Medicine
Glutathione
Thiophanate
Molecular biology
Fungicides
Industrial
0104 chemical sciences
Oxidative Stress
medicine.anatomical_structure
Liver
chemistry
Toxicity
biology.protein
Reactive Oxygen Species
Oxidation-Reduction
Injections
Intraperitoneal
Oxidative stress
Zdroj: Cellular and Molecular Biology. 63:20
ISSN: 1165-158X
Popis: The aim of this study was to elucidate the redox effects of Thiophanate methyl (MT) in the rat liver and kidney. Our results showed, after 3 days of MT injection (700 mg/kg), an increase in malondialdehyde (MDA), hydrogen peroxide and advanced oxidation protein products levels. Glutathione peroxidase and superoxide dismutase activities were also remarkably increased in the liver but decrease in the kidney. Glutathione and vitamin C values were significantly reduced. The changes in biochemical parameters were substantiated by histological and molecular data. A smear without ladder formation on agarose gel was shown, indicating random DNA degradation in the liver and the kidney of MT treated rats. The increase in cyclooxygenase-2 gene expression, marker of inflammation, and an increase in genes expression of glutathione peroxidase and superoxide dismutase in liver and their decrease in the kidney were also occurred after MT exposure. These data confirmed the pro-oxidant and genotoxic effects of this fungicide.
Databáze: OpenAIRE
Externí odkaz: