Great phenotypic and genetic variation among successive chronic Pseudomonas aeruginosa from a cystic fibrosis patient

Autor: José Manuel Azcona-Gutiérrez, Yolanda Sáenz, Carmen Lozano, Françoise Van Bambeke
Přispěvatelé: UCL - SSS/LDRI - Louvain Drug Research Institute
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Cystic Fibrosis
lcsh:Medicine
Drug resistance
Integron
medicine.disease_cause
Integrons
chemistry.chemical_compound
lcsh:Science
Multidisciplinary
biology
Quorum Sensing
General Medicine
Middle Aged
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
Pseudomonas aeruginosa
General Agricultural and Biological Sciences
Genotype
030106 microbiology
Porins
Virulence
Genetics and Molecular Biology
Microbial Sensitivity Tests
Opportunistic Infections
Serogroup
beta-Lactamases
Microbiology
03 medical and health sciences
Pyocyanin
Bacterial Proteins
Drug Resistance
Bacterial
medicine
Humans
Pseudomonas Infections
lcsh:R
Biofilm
Genetic Variation
Gene Expression Regulation
Bacterial
Clone Cells
Quorum sensing
030104 developmental biology
chemistry
Genes
Bacterial
Biofilms
Chronic Disease
General Biochemistry
biology.protein
Multilocus sequence typing
lcsh:Q
Multilocus Sequence Typing
Zdroj: PLOS ONE, Vol. 13, no. 9, p. e0204167 (2018)
PLoS ONE, Vol 13, Iss 9, p e0204167 (2018)
RIUR. Repositorio Institucional de la Universidad de La Rioja
instname
ISSN: 1932-6203
Popis: Background/Objectives Different adapted Pseudomonas aeruginosa morphotypes are found during chronic infections. Relevant biological determinants in P. aeruginosa successively isolated from a cystic fibrosis (CF) patient were analyzed in this work to gain insight into P. aeruginosa heterogeneity during chronic infection. Methods Seventeen P. aeruginosa isolates collected from a patient over a 3 year period were included, 5 small colony variants (SCV) and 12 mucoids. The following analyses were performed: Pulsed-Field-Gel-Electrophoresis (PFGE)/Multilocus- sequence-typing (MLST)/serotype, antimicrobial susceptibility, growth curves, capacity to form biofilm, pigment production, elastase activity, motility; presence/expression of virulence/quorum sensing genes, and identification of resistance mechanisms. Results All isolates had closely related PFGE patterns and belonged to ST412. Important phenotypic and genotypic differences were found. SCVs were more resistant to antimicrobials than mucoid isolates. AmpC hyperproduction and efflux pump activity were detected. Seven isolates contained two integrons and nine isolates only one integron. All SCVs showed the same OprD profile, while three different profiles were identified among mucoids. No amino acid changes were found in MutL and MutS. All isolates were slow-growing, generally produced high biofilm, had reduced their toxin expression and their quorum sensing, and showed low motility. Nevertheless, statistically significant differences were found among SCV and mucoid isolates. SCVs grew faster, presented higher biofilm formation and flicA expression; but produced less pyorubin and pyocyanin, showed lower elastase activity and rhlR, algD, and lasB expression than mucoid isolates. Conclusion These results help to understand the molecular behavior of chronic P. aeruginosa isolates in CF patients.
Databáze: OpenAIRE
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