The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest, and pronephric kidney
| Autor: | Brant M. Weinstein, Sung-Kook Hong, Nathan D. Lawson, Igor B. Dawid, Caroline E. Haldin, Neil A. Hukriede |
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| Rok vydání: | 2005 |
| Předmět: |
Embryo
Nonmammalian Molecular Sequence Data Mutant Morphogenesis Locus (genetics) Biology Kidney Animals Drosophila Proteins Cloning Molecular Eye Proteins Zebrafish Genetics Regulation of gene expression Mediator Complex Multidisciplinary Base Sequence Brain Gene Expression Regulation Developmental Neural crest Kidney metabolism Biological Sciences Zebrafish Proteins Cell sorting biology.organism_classification Cell biology Phenotype Neural Crest Mutation Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences. 102:18473-18478 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Mutation of the gene encoding the Mediator component thyroid hormone receptor-associated protein (TRAP)230/MED12 affects the development of multiple systems in zebrafish embryogenesis. We isolated two ethylnitrosourea-induced alleles in the gene encoding this protein and named the locus kohtalo ( kto ) after the homologous locus in Drosophila . Homozygous kto mutant zebrafish embryos show defects in brain, neural crest, and kidney development and die at ≈6 days postfertilization. In the affected tissues, differentiation is initiated and many cell type-specific genes are expressed, but there is a failure of morphogenesis and failure to complete differentiation. These results suggest that critical targets of TRAP230 function may include proteins important for cell mobility, cell sorting, and tissue assembly. |
| Databáze: | OpenAIRE |
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