Effect of Mouse Chromosome 13 Terminal Fragment on Liability to Catalepsy and Expression of Tryptophane Hydroxylase-2, Serotonin Transporter, and 5-HT1A Receptor Genes in the Brain
| Autor: | Nina K. Popova, Darya Bazovkina, Alexander V. Kulikov, Vladimir S. Naumenko, D. V. Osipova, V. Yu. Dee |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Congenic Locus (genetics) Tryptophan Hydroxylase Catalepsy General Biochemistry Genetics and Molecular Biology Mice Mice Inbred AKR medicine Animals Genetic Predisposition to Disease Receptor Gene Serotonin transporter Chromosome 13 Serotonin Plasma Membrane Transport Proteins biology Brain General Medicine medicine.disease Chromosomes Mammalian Molecular biology Receptor Serotonin 5-HT1A Mice Inbred CBA biology.protein 5-HT1A receptor |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 147:621-624 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | Congenic mice obtained by genome fragments transfer from one strain to another are a potent tool for studies of the molecular mechanisms of behavioral mutations. The 59-70 cM fragment of chromosome 13 containing the locus determining predisposition to freezing reaction (catalepsy) and the gene encoding 5-HT(1A) receptor were transferred from cataleptic CBA/Lac mice into the genome of catalepsy-resistant AKR/J mice. The impact of this fragment for the severity of catalepsy and expression of genes encoding tryptophane hydroxylase-2, serotonin transporter, and 5-HT(1A) receptor was studied. Half of mice of the resultant congenic AKR.CBA-D13Mit76 strain exhibited pronounced catalepsy, similarly to donor CBA animals. The expression of 5-HT(1A) receptor gene in the midbrain of AKR animals was significantly higher than in CBA. The level of 5-HT(1A) receptor mRNA in AKR.CBA-D13Mit76 animals was significantly higher than in the donor strain. Mice of parental AKR and CBA strains did not differ from each other and from AKR.CBA-D13Mit76 animals by the levels of tryptophane hydroxylase-2 and serotonin transporter genes mRNA. These data prove the location of catalepsy regulating gene in the distal fragment of chromosome 13. The recipient strain genome enhanced the expression of 5-HT(1A) receptor gene in the brain without modulating the expression of catalepsy gene. |
| Databáze: | OpenAIRE |
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