Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells
| Autor: | Susana Pedreiro, Joana Gomes, Hélder Trindade, Monia Pedrosa, Brígida Antunes, António Martinho, Tania Ribeiro, Artur Paiva, Francisco dos Santos, Paula Laranjeira |
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| Jazyk: | angličtina |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Interleukin 2 medicine.medical_specialty Stromal cell medicine.medical_treatment Medicine (miscellaneous) Bone Marrow Cells CD8-Positive T-Lymphocytes Biology Lymphocyte Activation Biochemistry Genetics and Molecular Biology (miscellaneous) Interferon-gamma Interleukin 21 Internal medicine medicine Humans Cytotoxic T cell Interferon gamma RNA Messenger IL-2 receptor Tumor Necrosis Factor-alpha Research Ionomycin Interleukin-17 Mesenchymal Stem Cells Cell Biology Middle Aged Molecular biology Coculture Techniques Endocrinology Cytokine Leukocytes Mononuclear Cytokines Interleukin-2 Tetradecanoylphorbol Acetate Molecular Medicine Female CD8 medicine.drug |
| Zdroj: | Stem Cell Research & Therapy |
| ISSN: | 1757-6512 |
| DOI: | 10.1186/scrt537 |
| Popis: | Introduction The different distribution of T cells among activation/differentiation stages in immune disorders may condition the outcome of mesenchymal stromal cell (MSC)-based therapies. Indeed, the effect of MSCs in the different functional compartments of T cells is not completely elucidated. Methods We investigated the effect of human bone marrow MSCs on naturally occurring peripheral blood functional compartments of CD4+ and CD8+ T cells: naive, central memory, effector memory, and effector compartments. For that, mononuclear cells (MNCs) stimulated with phorbol myristate acetate (PMA) plus ionomycin were cultured in the absence/presence of MSCs. The percentage of cells expressing tumor necrosis factor-alpha (TNF-α), interferon gamma (IFNγ), and interleukin-2 (IL-2), IL-17, IL-9, and IL-6 and the amount of cytokine produced were assessed by flow cytometry. mRNA levels of IL-4, IL-10, transforming growth factor-beta (TGF-β), and cytotoxic T-lymphocyte-associated protein 4 (CTLA4) in purified CD4+ and CD8+ T cells, and phenotypic and mRNA expression changes induced by PMA + ionomycin stimulation in MSCs, were also evaluated. Results MSCs induced the reduction of the percentage of CD4+ and CD8+ T cells producing TNF-α, IFNγ, and IL-2 in all functional compartments, except for naive IFNγ+CD4+ T cells. This inhibitory effect differentially affected CD4+ and CD8+ T cells as well as the T-cell functional compartments; remarkably, different cytokines showed distinct patterns of inhibition regarding both the percentage of producing cells and the amount of cytokine produced. Likewise, the percentages of IL-17+, IL-17+TNF-α+, and IL-9+ within CD4+ and CD8+ T cells and of IL-6+CD4+ T cells were decreased in MNC-MSC co-cultures. MSCs decreased IL-10 and increased IL-4 mRNA expression in stimulated CD4+ and CD8+ T cells, whereas TGF-β was reduced in CD8+ and augmented in CD4+ T cells, with no changes for CTLA4. Finally, PMA + ionomycin stimulation did not induce significant alterations on MSCs phenotype but did increase indoleamine-2,3-dioxygenase (IDO), inducible costimulatory ligand (ICOSL), IL-1β, IL-8, and TNF-α mRNA expression. Conclusions Overall, our study showed that MSCs differentially regulate the functional compartments of CD4+ and CD8+ T cells, which may differentially impact their therapeutic effect in immune disorders. Furthermore, the influence of MSCs on IL-9 expression can open new possibilities for MSC-based therapy in allergic diseases. Electronic supplementary material The online version of this article (doi:10.1186/scrt537) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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