Inhibition of B16 melanoma growth and metastasis in C57BL mice by vaccination with a syngeneic endothelial cell line
| Autor: | Naomi Yamashita, Takashi Nakaoka, Kenta Yoshiura, Naohide Yamashita, Toshihide Nishishita |
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| Rok vydání: | 2009 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Lung Neoplasms Angiogenesis medicine.medical_treatment Melanoma Experimental Cancer Vaccines Immunotherapy Adoptive lcsh:RC254-282 Cell Line Metastasis Mice Subcutaneous injection Antibody Specificity Cell Line Tumor Animals Medicine Neovascularization Pathologic business.industry Research Melanoma Endothelial Cells Immunotherapy lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Acquired immune system medicine.disease Vaccine therapy Mice Inbred C57BL Endothelial stem cell Oncology business |
| Zdroj: | Journal of Experimental & Clinical Cancer Research, Vol 28, Iss 1, p 13 (2009) Journal of Experimental & Clinical Cancer Research : CR |
| ISSN: | 1756-9966 |
| DOI: | 10.1186/1756-9966-28-13 |
| Popis: | Background Key role of angiogenesis in tumor growth and metastasis based on accumulating evidence and recent progress of immunotherapy have led us to investigate vaccine therapy targeting tumor angiogenesis. Methods C57BL/6J mice were vaccinated with a syngeneic endothelial cell line Tpit/E by subcutaneous injection once a week. Prior to ninth vaccination, the mice were challenged with B16/F10 melanoma cells by subcutaneous inoculation on the back for the tumor growth model or by tail venous injection for the lung metastasis model. Development of subcutaneous tumor and lung metastasis was monitored by computed tomography scanning, which enabled accurate evaluation with the minimized sacrifice of mice. Results Vaccination with Tpit/E cells inhibited subcutaneous tumor growth and appearance of lung metastasis compared to control. Survival period was elongated in the Tpit/E vaccination in both of the two models. We also obtained hybridomas secreting specific antibodies to Tpit/E cells from a mouse vaccinated with the cells, indicating that specific immune response to the syngeneic endothelial cells was elicited. Conclusion These results suggest that vaccination with an autologous endothelial cell line may be effective against melanoma. |
| Databáze: | OpenAIRE |
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