Normalization of keratinocyte-type integrins during the establishment of the oral mucosa phenotype in vitro
| Autor: | Pascal Tomakidi, Dirk Breitkreutz, G. Komposch, Annette Kohl |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Keratinocytes
Integrins Pathology medicine.medical_specialty Time Factors Cell Integrin Gingiva Integrin alpha6 Organ Culture Techniques Antigens CD In vivo Keratin medicine Humans Oral mucosa Cells Cultured chemistry.chemical_classification biology Integrin beta1 Mouth Mucosa Cell Differentiation General Medicine Fibroblasts Integrin alphaV Coculture Techniques Epithelium In vitro Cell biology medicine.anatomical_structure chemistry biology.protein Keratins Anatomy Keratinocyte Developmental Biology |
| Zdroj: | Annals of Anatomy - Anatomischer Anzeiger. 181:127-132 |
| ISSN: | 0940-9602 |
| DOI: | 10.1016/s0940-9602(99)80118-6 |
| Popis: | Summary In stratified epithelia, integrins play a fundamental role in mediating basal cell attachment to a variety of extracellular matrix molecules. To assess whether keratinocyte-specific integrins are expressed in a similar way as in the normal situation also under in vivo related conditions, we processed oral mucosa equivalents consisting of keratinocytes and fibroblasts from non-cornified gingiva. In this model histomorphology, the expression of differentiation-specific keratins and keratinocyte-type integrins exhibited similarity to the tissue of origin. The stages of tissue normalization were assessed on frozen sections by indirect immunofluorescence. The initial activated stage (1 week) was characterized by ( i ) incomplete epithelial organization and a weak presence of the suprabasal mucosa type keratin K4, ( ii ) diffuse expression of the integrin chains β 1 and α 6 and ( iii ) abundance of the wound healing-associated integrin α v throughout the whole epithelium. After 2 weeks, the increase in epithelial organization was characterized by ( i ) the presence of a basal and suprabasal cell compartment, ( ii ) extension of K4 in the suprabasal compartment, ( iii ) extended expression of the keratinocyte integrins β 1 and α 6 and ( iv ) concentration of α v integrin underneath basal cells. Further normalization of tissue architecture was indicated by ( i ) a slight increase in K4 extension, ( ii ) appearance of keratinocyte integrins β 1 and α 6 in basal and parabasal cells and ( iii ) interruption of the band-like α v integrin immunolocalization at the subepithelial site. The findings in the in vitro model system indicate that these oral mucosa equivalents exhibit similarities to the in vivo situation of non-cornified gingiva, thus rendering them a suitable model for the assessment of stages during epithelial reconstruction or in vivo relevant studies on material effects. |
| Databáze: | OpenAIRE |
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