INVESTIGATING MEMBRANE MORPHOLOGY AND QUANTITY OF IMMOBILIZED PROTEIN FOR THE DEVELOPMENT OF LATERAL FLOW IMMUNOASSAY

Autor:	S.R. Abd Shukor, Siew Chun Low, Abdul Latif Ahmad, Asma Ismail
Rok vydání:	2012
Předmět:	Immobilized protein Morphology (linguistics) Clinical Biochemistry Immunology Membrane morphology chemistry.chemical_compound Collodion medicine Animals Immunology and Allergy Ponceau S Immunoassay Chromatography medicine.diagnostic_test Chemistry Membranes Artificial Serum Albumin Bovine Staining Medical Laboratory Technology Immobilized Proteins Membrane Microscopy Electron Scanning Cattle Muramidase
Zdroj:	Journal of Immunoassay and Immunochemistry. 33:48-58
ISSN:	1532-4230 1532-1819
DOI:	10.1080/15321819.2011.591479
Popis:	This study was aimed at gaining a quantitative understanding of the effect of protein quantity and membrane pore structure on protein immobilization. The concentration of immobilized protein was measured by staining with Ponceau S and measuring its color intensity. In this study, both membrane morphology and the quantity of deposited protein significantly influenced the quantity of protein immobilization on the membrane surface. The sharpness and intensity of the red protein spots varied depending on the membrane pore structure, indicating a dependence of protein immobilization on this factor. Membranes with smaller pores resulted in a higher color density, corresponding to enhanced protein immobilization and an increased assay sensitivity level. An increased of immobilized volume has a significant jagged outline on the protein spot but, conversely, no difference in binding capacity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3ff5111c5947ee9647db4fb69abc6e8 https://doi.org/10.1080/15321819.2011.591479 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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