Induction of laryngeal cancer cell death by Ent-11-hydroxy-15-oxo-kaur-16-en-19-oic acid
| Autor: | Xianling Gong, Chun Shan Lo, Yi Feng Deng, Kefeng Wu, George G. Chen, Vivian Wai Yan Lui, C. Andrew van Hasselt, Michael C. F. Tong, Yingnian Lu, Nian Ci Liang, Alexander C. Vlantis |
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| Rok vydání: | 2010 |
| Předmět: |
Programmed cell death
Pathology medicine.medical_specialty Transcription Genetic Survivin Blotting Western Down-Regulation Apoptosis Inhibitor of Apoptosis Proteins Annexin Cell Line Tumor Medicine Humans Cyclin D1 Laryngeal Neoplasms Cell Proliferation bcl-2-Associated X Protein Dose-Response Relationship Drug business.industry Caspase 3 Tumor Necrosis Factor-alpha NF-kappa B Cancer medicine.disease Molecular biology I-kappa B Kinase Up-Regulation ErbB Receptors IκBα Otorhinolaryngology Cell culture Cyclooxygenase 2 Cancer cell Diterpenes Poly(ADP-ribose) Polymerases business Microtubule-Associated Proteins Drugs Chinese Herbal |
| Zdroj: | Headneck. 32(11) |
| ISSN: | 1097-0347 |
| Popis: | Background. Ent-11-hydroxy-15-oxo-kaur-16-en-19-oic acid (5F) is known to exhibit antitumor activity, but its mechanism is not completely understood. 5F has not been tested in laryngeal cancer. Methods. Two laryngeal cancer cell lines were treated with 5F. Cell death was analyzed by MTT [3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide] and Annexin V assay. Nuclear factor kappa beta (NF-κB)– and apoptosis-related molecules were examined. Results. 5F induced laryngeal cancer cell death in a dose-dependent manner. The Annexin V assay and the measurement of cleavage of procaspase-3 and poly(ADP-ribose) polymerase demonstrated that the 5F-induced cell death was mainly apoptotic. 5F slightly reduced the basal level of NF-κB, but significantly suppressed the inducible NF-κB by reducing its transcriptional activity, protecting its inhibitory subunit IκBα from degradation, and suppressing its level in the nucleus. 5F also inhibited pro-proliferative and anti-apoptotic molecules but promoted pro-apoptotic Bax. Conclusions. 5F induces apoptosis of laryngeal cancer cells by inhibiting NF-κB activation/induction, suppressing pro-proliferative and anti-apoptotic molecules, and promoting pro-apoptotic Bax. © 2010 Wiley Periodicals, Inc. Head Neck, 2010 |
| Databáze: | OpenAIRE |
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