Resveratrol-induced changes of the human adipocyte secretion profile
| Autor: | Anja Rosenow, Pamela Fischer-Posovszky, Sonja Kallendrusch, Edwin C. M. Mariman, Johan Renes, Jean-Paul Noben, Johan W. E. Jocken |
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| Přispěvatelé: | Humane Biologie, RS: NUTRIM - R4 - Gene-environment interaction, RS: NUTRIM - R1 - Metabolic Syndrome, Rosenow, Anja, NOBEN, Jean-Paul, Jocken, Johan, Kallendrusch, Sonja, Fischer-Posovszky, Pamela, MARIMAN, Edwin C.M., Renes, Johan |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Proteome Lipolysis Calorie restriction human adipocytes RSV adipokines lipolysis 2-DE LC-MS/MS Adipokine White adipose tissue Resveratrol Biology Biochemistry Cell Line chemistry.chemical_compound Adipokines Tandem Mass Spectrometry Adipocyte Internal medicine Stilbenes medicine Adipocytes Humans Secretion Electrophoresis Gel Two-Dimensional Biochemical Research Methods 2-DE LC MS/MS Adiponectin Proteins Reproducibility of Results General Chemistry Endocrinology chemistry Chromatography Liquid |
| Zdroj: | Journal of Proteome Research, 11(9), 4733-4743. American Chemical Society |
| ISSN: | 1535-3907 1535-3893 |
| Popis: | Enlarged white adipose tissue (WAT) is a feature of obesity and leads to changes in its paracrine and endocrine function. Dysfunction of WAT cells is associated with obesity-associated disorders like type 2 diabetes and cardiovascular diseases. Resveratrol (RSV), a natural polyphenolic compound, mimics beneficial effects of calorie restriction. As such, RSV seems a promising therapeutic target for obesity-associated disorders. The effect of RSV on the human adipokine profile is still elusive. Therefore, a proteomic study together with bioinformatical analysis was performed to investigate the effect of RSV on the secretion profile of mature human SGBS adipocytes. RSV incubation resulted in elevated basal glycerol release and reduced intracellular TG content. This increased intracellular lipolysis was accompanied by profound changes in the adipocyte secretion profile. Extracellular matrix proteins were down-regulated while processing proteins were mostly up-regulated after RSV treatment. Interestingly, RSV induced secretion of proteins protective against cellular stress and proteins involved in the regulation of apoptosis. Furthermore, we found a RSV-induced up-regulation of adiponectin and ApoE accompanied by a down-regulation of PAI-1 and PEDF secretion which may improve anti-inflammatory processes and increased insulin sensitivity. These effects may contribute to alleviate obesity-induced metabolic complications. In addition, two novel RSV-regulated adipocyte-secreted proteins were identified. |
| Databáze: | OpenAIRE |
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