Evaluation of Two HIV Rapid Diagnostic Tests in a Context of Strains' Genetic Diversity in Mali
| Autor: | Oumar Dolo, F. Traoré, Souleymane Diallo, Vincent Calvez, Robert L. Murphy, Almoustapha Issiaka Maiga, Josue Togo, Anne-Geneviève Marcelin, Mariam Sylla, Bourahima Kone, Samba Adama Sangare, Mamoudou Maiga |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male Adolescent Epidemiology Immunology Human immunodeficiency virus (HIV) Context (language use) HIV Infections HIV Antibodies medicine.disease_cause Mali Sensitivity and Specificity World health West africa 03 medical and health sciences Young Adult 0302 clinical medicine Predictive Value of Tests Virology Environmental health medicine Humans Serologic Tests 030212 general & internal medicine Child Retrospective Studies Genetic diversity Rapid diagnostic test virus diseases Diagnostic test Genetic Variation Middle Aged 3. Good health 030104 developmental biology Infectious Diseases Geography Cross-Sectional Studies Child Preschool HIV-2 HIV-1 Female Reagent Kits Diagnostic |
| Zdroj: | AIDS research and human retroviruses. 35(2) |
| ISSN: | 1931-8405 |
| Popis: | The rapid diagnostic tests used in most resource-limited countries offer little specificity in the differentiation of HIV-1, HIV-2, and HIV-1 + 2. World Health Organization (WHO) does periodically evaluate rapid tests in use in the South Countries. Despite the prequalification from WHO, it is necessary at local level to conduct comparative studies between the available tests. To do this in Mali, we conducted a cross-sectional study at the University Teaching Hospital Gabriel Touré and the Research and Training Center (SEREFO) of Bamako on 172 samples from retrospective and prospective studies. The goal of this project was to compare the following rapid tests: “HIV TRI-DOT” and OnSite HIV1/2 Ab Plus Combo Rapid Test for screening HIV-1 and HIV-2 to “ImmunoComb II BiSpot” (gold standard). The sensibility and specificity of “HIV TRI-DOT” for HIV-1 detection were 100% each [95% confidence interval (CI): 87.13–100 and 95.31–100]. Its performance for HIV-2 detection was 95.24% (95% CI: 84.21–98.68) (sensibility) and 100% (95% CI: 72.25–100) (specificity). The positive and negative predictive values were, respectively, 100% (95% CI: 91.24–100) and 83.33% (95% CI: 55.20–95.30). The sensibility and specificity of OnSite HIV1/2 Ab Plus Combo Rapid Test assay performance were 100% (95% CI: 87.13–100 and 95.31–100). Overall, OnSite HIV ½ Ab Combo Rapid Test proved to be a good test for warm countries and does not require refrigeration in these settings. Blood and its products can be used to perform the test, unlike “ImmunoComb II BiSpot” and “HIV TRI-DOT,” which must be kept cold and cannot be done with whole blood. |
| Databáze: | OpenAIRE |
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