The Transcriptional Repressor dMnt Is a Regulator of Growth in Drosophila melanogaster
| Autor: | Erin M. Flynn, Julie Secombe, Lenora W. M. Loo, Bruce A. Edgar, Leni Sue Carlos, John T. Little, Pei Feng Cheng, Robert N. Eisenman, Cynthia Yost |
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| Rok vydání: | 2005 |
| Předmět: |
Time Factors
Transcription Genetic Recombinant Fusion Proteins Green Fluorescent Proteins Longevity Gene Expression Repressor Cell Separation DNA-binding protein Upstream activating sequence Two-Hybrid System Techniques Melanogaster Animals Body Size Drosophila Proteins Insulin Molecular Biology Transcription factor Alleles Phylogeny Cell Proliferation Glutathione Transferase Genetics Models Genetic biology Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Reverse Transcriptase Polymerase Chain Reaction DNA Cell Biology Flow Cytometry biology.organism_classification Immunohistochemistry Protein Structure Tertiary DNA-Binding Proteins Repressor Proteins Alternative Splicing Drosophila melanogaster Phenotype Mutation Dimerization Corepressor Drosophila Protein Protein Binding Transcription Factors |
| Zdroj: | Molecular and Cellular Biology. 25:7078-7091 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.25.16.7078-7091.2005 |
| Popis: | The Myc-Max-Mad/Mnt network of transcription factors has been implicated in oncogenesis and the regulation of proliferation in vertebrate cells. The identification of Myc and Max homologs in Drosophila melanogaster has demonstrated a critical role for dMyc in cell growth control. In this report, we identify and characterize the third member of this network, dMnt, the sole fly homolog of the mammalian Mnt and Mad family of transcriptional repressors. dMnt possesses two regions characteristic of Mad and Mnt proteins: a basic helix-loop-helix-zipper domain, through which it dimerizes with dMax to form a sequence-specific DNA binding complex, and a Sin-interacting domain, which mediates interaction with the dSin3 corepressor. Using the upstream activation sequence/GAL4 system, we show that expression of dMnt results in an inhibition of cellular growth and proliferation. Furthermore, we have generated a dMnt null allele, which results in flies with larger cells, increased weight, and decreased life span compared to wild-type flies. Our results demonstrate that dMnt is a transcriptional repressor that regulates D. melanogaster body size. |
| Databáze: | OpenAIRE |
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