Simple silicone chamber system for in vitro three-dimensional skeletal muscle tissue formation
| Autor: | K.P. Goetsch, Carola U. Niesler, Celia Snyman, Kathryn H. Myburgh |
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| Rok vydání: | 2013 |
| Předmět: |
Scaffold
Pathology medicine.medical_specialty Physiology business.industry Myogenesis Regeneration (biology) Skeletal muscle three-dimensional assays in vitro skeletal muscle tissue Matrix (biology) chemistry.chemical_compound Silicone medicine.anatomical_structure chemistry Tissue engineering tissue engineering Physiology (medical) hydrogel constructs Methods Article Medicine Myocyte business Biomedical engineering |
| Zdroj: | Frontiers in Physiology |
| ISSN: | 1664-042X |
| DOI: | 10.3389/fphys.2013.00349 |
| Popis: | Bioengineering skeletal muscle often requires customized equipment and intricate casting techniques. One of the major hurdles when initially trying to establish in vitro tissue engineered muscle constructs is the lack of consistency across published methodology. Although this diversity allows for specialisation according to specific research goals, lack of standardisation hampers comparative efforts. Differences in cell type, number and density, variability in matrix and scaffold usage as well as inconsistency in the distance between and type of adhesion posts complicates initial establishment of the technique with confidence. We describe an inexpensive, but readily adaptable silicone chamber system for the generation of skeletal muscle constructs that can readily be standardized and used to elucidate myoblast behaviour in a three-dimensional space. Muscle generation, regeneration and adaptation can also be investigated in this model, which is more advanced than differentiated myotubes. |
| Databáze: | OpenAIRE |
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