The In Ovo Delivery of CpG Oligonucleotides Protects against Infectious Bronchitis with the Recruitment of Immune Cells into the Respiratory Tract of Chickens

Autor: Aruna Amarasinghe, Mohamed Sarjoon Abdul-Cader, Susantha Gomis, Guido van Marle, Mohamed Faizal Abdul-Careem, Markus Czub, Upasama De Silva Senapathi
Rok vydání: 2018
Předmět:
0301 basic medicine
CD4-Positive T-Lymphocytes
infectious bronchitis virus
animal structures
CD4+ cell
040301 veterinary sciences
Respiratory System
lcsh:QR1-502
Infectious bronchitis virus
Biology
CD8-Positive T-Lymphocytes
In ovo
CD8α+ cell
lcsh:Microbiology
0403 veterinary science
03 medical and health sciences
Interferon-gamma
Immune system
Adjuvants
Immunologic
Interferon
Virology
medicine
Animals
avian macrophage
CpG oligonucleotide
Receptor
Poultry Diseases
Cluster of differentiation
Macrophages
Communication
04 agricultural and veterinary sciences
respiratory system
Survival Analysis
3. Good health
Up-Regulation
030104 developmental biology
medicine.anatomical_structure
Infectious Diseases
CpG site
Oligodeoxyribonucleotides
Immunology
embryonic structures
Coronavirus Infections
Chickens
medicine.drug
Respiratory tract
Zdroj: Viruses
Viruses, Vol 10, Iss 11, p 635 (2018)
ISSN: 1999-4915
Popis: The in ovo delivery of cytosine-guanosine (CpG) oligodeoxynucleotides (ODNs) protects chickens against many bacterial and viral infections, by activating the toll-like receptor (TLR)21 signaling pathway. Although the delivery of CpG ODNs in ovo at embryo day (ED) 18 has been shown to reduce infectious bronchitis virus (IBV) loads in embryonic chicken lungs pre-hatch, whether in ovo delivered CpG ODNs are capable of protecting chickens against a post-hatch challenge is unknown. Thus, our objectives were to determine the protective effect of the in ovo delivery of CpG ODNs at ED 18 against IBV infection encountered post-hatch and, then, to investigate the mechanisms of protection. We found significantly higher survival rates and reduced IBV infection in the chickens following the pre-treatment of the ED 18 eggs with CpG ODNs. At 3 days post infection (dpi), we found an increased recruitment of macrophages, cluster of differentiation (CD)8α+ and CD4+ T lymphocytes, and an up-regulation of interferon (IFN)-γ mRNA in the respiratory tract of the chickens. Overall, it may be inferred that CpG ODNs, when delivered in ovo, provide protection against IBV infection induced morbidity and mortality with an enhanced immune response.
Databáze: OpenAIRE
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