A novel class of gene controlling virulence in plant pathogenic ascomycete fungi
| Autor: | Barbara Robbertse, Shunwen Lu, O. C. Yoder, Bee-Na Lee, Alice C. L. Churchill, B. Gillian Turgeon, Scott Kroken |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Avena
Molecular Sequence Data Virulence Cochliobolus heterostrophus Polymerase Chain Reaction Virulence factor Microbiology Ascomycota Animals Humans Cloning Molecular Pathogen Phylogeny Triticum Cochliobolus Multidisciplinary biology fungi Membrane Proteins food and beverages Plants Biological Sciences biology.organism_classification Gibberella zeae Glucosyltransferases Mutagenesis Cochliobolus victoriae Schizosaccharomyces pombe Proteins Gene Deletion |
| Zdroj: | Proceedings of the National Academy of Sciences. 100:5980-5985 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0931375100 |
| Popis: | Insertional mutants of the fungal maize pathogen Cochliobolus heterostrophus were screened for altered virulence. One mutant had 60% reduction in lesion size relative to WT but no other detectable change in phenotype. Analysis of sequence at the insertion site revealed a gene ( CPS1 ) encoding a protein with two AMP-binding domains. CPS1 orthologs were detected in all Cochliobolus spp. examined, in several other classes of ascomycete fungi, and in animals but not in basidiomycete fungi, bacteria, or plants. Phylogenetic analysis suggested that CPS1 represents a previously undescribed subset of adenylate-forming enzymes that have diverged from certain acyl-CoA ligases, which in bacteria are involved in biosynthesis of nonribosomal peptides or polyketide/peptide hybrids. Disruption of CPS1 caused reduced virulence of both race T and race O of C. heterostrophus on maize, of Cochliobolus victoriae on oats, and of Gibberella zeae on wheat. These results suggest that CPS1 functions as a general fungal virulence factor in plant pathogenic ascomycetes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|