Alteration of Endothelial Cell Monolayer Integrity Triggers Resynthesis of Vascular Endothelium Cadherin
| Autor: | Robin Scaife, Yolande Genoux, Evelyne Concord, Danielle Gulino, Eric Sulpice, Elisabeth Delachanal, Thierry Vernet, Monica Alemany, Blandine Morand, Marie-Odile Valiron |
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| Rok vydání: | 1998 |
| Předmět: |
Cell Membrane Permeability
Endothelium Molecular Sequence Data Biochemistry Antigens CD Cell Adhesion medicine Extracellular Humans Amino Acid Sequence RNA Messenger Molecular Biology Cells Cultured DNA Primers Base Sequence biology Cadherin Cell Biology Cadherins In vitro Cell biology Endothelial stem cell medicine.anatomical_structure Microscopy Fluorescence Polyclonal antibodies biology.protein Endothelium Vascular Antibody Function (biology) |
| Zdroj: | Journal of Biological Chemistry. 273:29786-29793 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.273.45.29786 |
| Popis: | Although cadherins appear to be necessary for proper cell-cell contacts, the physiological role of VE-cadherin (vascular endothelium cadherin) in adult tissue has not been clearly determined. To shed some light on this question, we have disturbed the adhesive function of VE-cadherin in human endothelial cell culture using a polyclonal anti-VE-cadherin antibody. This antibody disrupts confluent endothelial cell monolayers in vitro and transiently generates numerous gaps at cell-cell junctions. The formation of these gaps correlates with a reversible increase in the monolayer permeability. We present evidence that destruction of the homotypic interactions between the extracellular domains of VE-cadherin induces a rapid resynthesis of VE-cadherin, leading to restoration of endothelial cell-cell contacts. The expression of new molecules of VE-cadherin correlates with a modest but significant increase in VE-cadherin mRNA synthesis. Altogether, these results establish a critical role for VE-cadherin in the maintenance and restoration of endothelium integrity. |
| Databáze: | OpenAIRE |
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