Does high mitochondrial efficiency carry an oxidative cost? The case of the African pygmy mouse (Mus mattheyi)
| Autor: | Frédéric Veyrunes, Damien Roussel, Anne-Cécile Durieux, Yann Voituron, Damien Freyssenet, Mélanie Boël |
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| Přispěvatelé: | Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Inter-University Laboratory of Human Movement Biology, University of Lyon, Saint-Etienne, France, parent |
| Rok vydání: | 2021 |
| Předmět: |
Bioenergetics
Physiology [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Oxidative phosphorylation Mitochondrion medicine.disease_cause Biochemistry Oxidative Phosphorylation 03 medical and health sciences Mice 0302 clinical medicine Oxygen Consumption Species Specificity medicine Animals Muscle Skeletal Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology chemistry.chemical_classification 0303 health sciences Reactive oxygen species Mus mattheyi biology ATP synthase Chemistry Skeletal muscle Hydrogen Peroxide biology.organism_classification Cell biology Mitochondria Muscle Adenosine Diphosphate Mice Inbred C57BL Oxidative Stress medicine.anatomical_structure biology.protein Reactive Oxygen Species 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology, Elsevier, 2022, 264, pp.111111. ⟨10.1016/j.cbpa.2021.111111⟩ |
| ISSN: | 1531-4332 1095-6433 |
| DOI: | 10.1016/j.cbpa.2021.111111⟩ |
| Popis: | Skeletal muscle mitochondria of the African pygmy mouse Mus mattheyi exhibit markedly reduced oxygen consumption and ATP synthesis rates but a higher mitochondrial efficiency than what would be expected from allometric trends. In the present study, we assessed whether such reduction of mitochondrial activity in M. mattheyi can limit the oxidative stress associated with an increased generation of mitochondrial reactive oxygen species. We conducted a comparative study of mitochondrial oxygen consumption, H2O2 release, and electron leak (%H2O2/O) in skeletal muscle mitochondria isolated from the extremely small African pygmy mouse (M. mattheyi, ~5 g) and Mus musculus, which is a larger Mus species (~25 g). Mitochondria were energized with pyruvate, malate, and succinate, after which fluxes were measured at different steady-state rates of oxidative phosphorylation. Overall, M. mattheyi exhibited lower oxidative activity and higher electron leak than M. musculus, while the H2O2 release did not differ significantly between these two Mus species. We further found that the high coupling efficiency of skeletal muscle mitochondria from M. mattheyi was associated with high electron leak. Nevertheless, data also show that, despite the higher electron leak, the lower mitochondrial respiratory capacity of M. mattheyi limits the cost of a net increase in H2O2 release, which is lower than that expected for a mammals of this size. |
| Databáze: | OpenAIRE |
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