Engineering Ashbya gossypii strains for de novo lipid production using industrial by‐products
| Autor: | José L. Revuelta, Rodrigo Ledesma-Amaro, Alberto Jiménez, Rubén M. Buey, Patricia Lozano-Martínez |
|---|---|
| Přispěvatelé: | Metabolic Engineering Group, Departamento de Microbiologia y Genetica, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, BASF, Spanish Ministerio de Economia y Competitividad, [BIO2014-56930-P], Luis Revuelta, Jose |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microorganism [SDV]Life Sciences [q-bio] 030106 microbiology Industrial Waste Bioengineering Biolipid Biology Applied Microbiology and Biotechnology Biochemistry Eremothecium Metabolic engineering 03 medical and health sciences Waste Management Lipid biosynthesis Molasses Sugar Research Articles Biotransformation 2. Zero hunger Downstream processing Lipid metabolism Lipid Metabolism Culture Media Glucose Metabolic Engineering Fermentation Biotechnology Research Article |
| Zdroj: | Microbial Biotechnology Microbial Biotechnology, Wiley, 2017, 10 (2), pp.425-433. ⟨10.1111/1751-7915.12487⟩ Microbial Biotechnology 2 (10), 425-433. (2017) |
| ISSN: | 1751-7915 |
| DOI: | 10.1111/1751-7915.12487⟩ |
| Popis: | Summary Ashbya gossypii is a filamentous fungus that naturally overproduces riboflavin, and it is currently exploited for the industrial production of this vitamin. The utilization of A. gossypii for biotechnological applications presents important advantages such as the utilization of low‐cost culture media, inexpensive downstream processing and a wide range of molecular tools for genetic manipulation, thus making A. gossypii a valuable biotechnological chassis for metabolic engineering. A. gossypii has been shown to accumulate high levels of lipids in oil‐based culture media; however, the lipid biosynthesis capacity is rather limited when grown in sugar‐based culture media. In this study, by altering the fatty acyl‐CoA pool and manipulating the regulation of the main ∆9 desaturase gene, we have obtained A. gossypii strains with significantly increased (up to fourfold) de novo lipid biosynthesis using glucose as the only carbon source in the fermentation broth. Moreover, these strains were efficient biocatalysts for the conversion of carbohydrates from sugarcane molasses to biolipids, able to accumulate lipids up to 25% of its cell dry weight. Our results represent a proof of principle showing the promising potential of A. gossypii as a competitive microorganism for industrial biolipid production using cost‐effective feed stocks. |
| Databáze: | OpenAIRE |
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