Non invasive backscattering light to detection of endothelial cells activity for graft sorting: Proof of consent
| Autor: | Emmanuel Bergeret, Laure Siozade Lamoine, Marion Gil, Laetitia Abel Tiberini, Carole Deumié, Gaëlle Georges |
|---|---|
| Přispěvatelé: | DiMABio (DiMABio), Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique de Clermont (LPC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011), Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Aix Marseille Université (AMU) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pump activity
Endothelium Endothelial cells 0206 medical engineering Cell Scattering light 02 engineering and technology Cornea 03 medical and health sciences 0302 clinical medicine medicine Diagnostic Process (anatomy) [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics] Chemistry Non invasive Sorting Graft sorting QC350-467 Optics. Light 020601 biomedical engineering Atomic and Molecular Physics and Optics medicine.anatomical_structure 030221 ophthalmology & optometry Entire cell Biomedical engineering |
| Zdroj: | Results in Optics Results in Optics, 2021, 4, pp.100082. ⟨10.1016/j.rio.2021.100082⟩ Results in Optics, Vol 4, Iss, Pp 100082-(2021) Results in Optics, Elsevier, 2021, 4, pp.100082. ⟨10.1016/j.rio.2021.100082⟩ |
| ISSN: | 2666-9501 |
| Popis: | International audience; In tissue bank, one of the key steps in the corneal grafts sorting is the analysis of the deepest cell layer of the cornea, the endothelium. This analysis consists of counting the density of living cells using trypan blue staining. However, this dye is toxic and the measurement is localized. Then no information is given on the cell's viability of the entire cell layer. This preliminary study proposes to track the kinetics of variation of scattered light during the deturgescence process. The backscattered light of two different sets of cornea, with and without endothelial cells, are measured and the decrease of this parameter as a function of the deturgescence time are different. This noninvasive technique could be used as a criterion for attesting the presence or absence of endothelial cells. These specificities make it possible to preserve the endothelial layer compared to the techniques. The ultimate objective would be to use this measurement as a sorting criterion characterizing endothelial pump activity. |
| Databáze: | OpenAIRE |
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