Manganese-enhanced MRI Offers Correlation with Severity of Spinal Cord Injury in Experimental Models
| Autor: | Gregory H. Turner, Nicholas Theodore, Arpan A. Patel, Jason A. Kaufman, M. Yashar S. Kalani, Evgenii Belykh, Nikolay L. Martirosyan, Mark C. Preul |
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| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Cord medicine.medical_treatment Manganese-enhanced MRI Spinal cord injury Article 030218 nuclear medicine & medical imaging Lesion 03 medical and health sciences 0302 clinical medicine Magnetic resonance imaging Fractional anisotropy medicine Radiology Nuclear Medicine and imaging Manganese Spinal cord medicine.diagnostic_test business.industry Laminectomy medicine.disease Surgery medicine.anatomical_structure Diffusion tensor imaging Neurology (clinical) medicine.symptom business Nuclear medicine 030217 neurology & neurosurgery Diffusion MRI |
| Zdroj: | The Open Neuroimaging Journal |
| ISSN: | 1874-4400 |
| Popis: | Background: Spinal cord injuries (SCI) are clinically challenging, because neural regeneration after cord damage is unknown. In SCI animal models, regeneration is evaluated histologically, requiring animal sacrifice. Noninvasive techniques are needed to detect longitudinal SCI changes. Objective: To compare manganese-enhanced magnetic resonance imaging (MRI [MEMRI]) in hemisection and transection of SCI rat models with diffusion tensor imaging (DTI) and histology. Methods: Rats underwent T9 spinal cord transection (n=6), hemisection (n=6), or laminectomy without SCI (controls, n=6). One-half of each group received lateral ventricle MnCl2 injections 24 hours later. Conventional DTI or T1-weighted MRI was performed 84 hours post-surgery. MEMRI signal intensity ratio above and below the SCI level was calculated. Fractional anisotropy (FA) measurements were taken 1 cm rostral to the SCI. The percentage of FA change was calculated 10 mm rostral to the SCI epicenter, between FA at the dorsal column lesion normalized to a lateral area without FA change. Myelin load (percentage difference) among groups was analyzed by histology. Results: In transection and hemisection groups, mean MEMRI ratios were 0.62 and 0.87, respectively, versus 0.99 in controls (P Conclusion: MEMERI results correlated to SCI severity measured by FA and myelin load. MEMRI is a useful noninvasive tool to assess neuronal damage after SCI. |
| Databáze: | OpenAIRE |
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