The Nuclear RNA-binding Protein RBSR1 Interactome in Trypanosoma cruzi
| Autor: | Samuel Goldenberg, Newton Medeiros Vidal, Bruna H. Marcon, Lysangela R. Alves, Sharon T. Martins, Halisson Tesseroli Miot, Juliane S. Malgarin, Fabricio K Marchini, Helisa H. Wippel |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Regulation of gene expression biology Trypanosoma cruzi Protozoan Proteins RNA-Binding Proteins RNA-binding protein 030108 mycology & parasitology biology.organism_classification Microbiology Interactome Cell biology Ribonomics 03 medical and health sciences Splicing factor 030104 developmental biology Amino Acid Sequence Small nucleolar RNA Phylogeny Nuclear localization sequence |
| Zdroj: | Journal of Eukaryotic Microbiology. 66:244-253 |
| ISSN: | 1066-5234 |
| Popis: | Trypanosoma cruzi, the etiological agent of Chagas disease, has been widely studied, reflecting both its medical importance and the particular features that make this pathogen an attractive model for basic biological studies. The repression of transcripts by messenger ribonucleoprotein (mRNP) complexes is an important pathway of post-transcriptional regulation in eukaryotes, including T. cruzi. RBSR1 is a serine-arginine (SR)-rich RNA-binding protein (RBP) in T. cruzi that contains one RNA-recognition motif (RRM); this protein has a primarily nuclear localization and is developmentally regulated, not being detected in metacyclic trypomastigotes. RBSR1 interacts with other RBPs, such as UBP1 and UBP2, and the nuclear SR-protein TRRM1. Phylogenetic analysis indicated that RBSR1 is orthologous to the human splicing factor SRSF7, what might indicate its possible involvement in pre-RNA processing. Accordingly, ribonomics data showed the enrichment of snoRNAs and snRNAs in the RBSR1 immunoprecipiatation complex, hence reinforcing the supposition that this protein might be involved in RNA processing in the nucleus. |
| Databáze: | OpenAIRE |
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