Morphological transition of Helicobacter pylori adapted to water
| Autor: | Hélder D. Silva, Nuno F. Azevedo, Maria João Vieira, Ricardo M. Fernandes, Carina Almeida, Ricardo F. Oliveira |
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| Přispěvatelé: | Faculdade de Medicina, Instituto de Ciências Biomédicas Abel Salazar, Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Peptidoglycan Biology Real-Time Polymerase Chain Reaction Microbiology 03 medical and health sciences Cell Wall Adaptation Genetics Supplementary data Science & Technology Helicobacter pylori Water Gene Expression Regulation Bacterial biology.organism_classification Adaptation Physiological 6. Clean water 3. Good health RNA Bacterial Phenotype 030104 developmental biology Genes Bacterial Microscopy Electron Scanning Morphological transition sense organs Water Microbiology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | To view the supplementary data that accompany this paper please visit the journal website at: www. futuremedicine.com /doi/full/10.2217/fmb-2016-0174. Aim: This study aims to investigate the morphological transition of Helicobacter pylori during adaptation to water. Materials \& methods: Different strains were adapted to water. Changes regarding cultivability and cellular morphology were recorded. Expression of 11 genes involved in H. pylori morphological changes was evaluated by real-time PCR. Results: H. pylori presented increased cultivability in water after adaptation. The permanent loss of the spiral shape was observed, but no transition into coccoid form has occurred. Expression levels of genes involved in peptidoglycan assembly of H. pylori 26695 have shown significant changes between adapted and nonadapted strains. Conclusion: Adaption to water favors the culturable phenotype and the morphological transition to the rod shape, into a process that implicates the peptidoglycan turnover. This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the project ‘Heliwater’ (PTDC/BIA-MIC/108811/2008), the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. No writing assistance was utilized in the production of this manuscript. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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