Local cytokine responses in Dictyocaulus viviparus infection
| Autor: | Jacqueline B. Matthews, J. Sales, Deborah R. Johnson |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Lung Diseases
Parasitic medicine.medical_treatment Antibodies Helminth Cattle Diseases Enzyme-Linked Immunosorbent Assay Biology Feces Immune system Immunity Parenchyma medicine Dictyocaulus Infections Mesenteric lymph nodes Animals Interferon gamma RNA Messenger Lung General Veterinary Reverse Transcriptase Polymerase Chain Reaction Interleukin General Medicine Dictyocaulus Trachea Cytokine medicine.anatomical_structure Immunology Cytokines Parasitology Cattle Female Lymph Lymph Nodes medicine.drug |
| Zdroj: | Veterinary parasitology. 128(3-4) |
| ISSN: | 0304-4017 |
| Popis: | The high degree of immunity induced by the bovine lungworm, Dictyocaulus viviparus, makes it an ideal model in which to study nematode-induced protective immune responses. Here, cytokine responses were measured over the course of an experimental infection of D. viviparus. Local cytokine messenger RNA (mRNA) transcripts were measured in lung parenchyma, tracheal rings and draining lymph nodes using semi-quantitative reverse transcriptase-polymerase chain reaction. Responses were measured in animals necropsied at 15, 22 and 43 days post-infection (DPI). The responses elicited at these time points were compared with cytokine levels observed in uninfected animals. Interleukin (IL)-4, IL-5, IL-10, IL-12p35, IL-13 and interferon gamma (IFNgamma) mRNA levels were measured in duplicate at each site. By 42 DPI, very few parasites were recovered, either from faeces or lungs. Transcripts of all cytokines increased in the lung parenchyma, tracheal rings and caudal mesenteric lymph nodes by 15 DPI. The response was rapid and peaked during the time of larval migration through the lungs. By 42 DPI, expression levels of most cytokines were reduced to levels similar to, or below, base line values measured in uninfected animals. Highest levels of IL-10, IL-12p35, IL-13 and IFNgamma transcript were measured in the bronchial lymph nodes of uninfected animals. IgG1 levels were negatively correlated with expression levels of all cytokines. The results demonstrate that a mixed cytokine response occurs over the course of a primary infection during which the parasites were eliminated by day 43 DPI. These results agree with those obtained for other helminths in cattle and challenge the hypothesis that polarised Th2 responses are essential for protection against nematodes in this species. These observations are important in the development of recombinant vaccines, particularly when considering adjuvant choice. |
| Databáze: | OpenAIRE |
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