Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy
| Autor: | Pierre J. Magistretti, Benjamin Rappaz, Christian Depeursinge, Etienne Cuche, Yves Emery, Pierre Marquet, Tristan Colomb |
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| Rok vydání: | 2005 |
| Předmět: |
Microscope
Holography Interferometric microscopy law.invention Imaging systems : Coherence imaging Mice Optical path Optics Holography : Aberration compensation law Image Interpretation Computer-Assisted Animals Microscopy Phase-Contrast Optical path length Cells Cultured Physics [MVD] Cerebral Cortex Neurons Image processing : Digital image processing business.industry Signal Processing Computer-Assisted Image Enhancement Computer-generated holography Atomic and Molecular Physics and Optics Medical optics and biotechnology : Coherence imaging Differential interference contrast microscopy Digital holographic microscopy Holography : Holography business Algorithms |
| Zdroj: | Optics letters. 30(5) |
| ISSN: | 0146-9592 |
| Popis: | We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsewhere [ Cuche , Appl. Opt.38, 6994 (1999)]. DHM images of living cells in culture are shown for what is to our knowledge the first time. They represent the distribution of the optical path length over the cell, which has been measured with subwavelength accuracy. These DHM images are compared with those obtained by use of the widely used phase contrast and Nomarski differential interference contrast techniques. |
| Databáze: | OpenAIRE |
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