Chemical probes for profiling fatty acid-associated proteins in living cells
| Autor: | Anuradha S. Raghavan, Howard C. Hang, Guillaume Charron, James Flexner |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Protein family
Clinical Biochemistry Pharmaceutical Science Molecular Probe Techniques Phospholipases A2 Cytosolic Cervix Uteri Fatty Acid-Binding Proteins Biochemistry Chemical synthesis Monocytes Jurkat Cells Mice Drug Discovery Molecule Animals Humans Molecular Biology chemistry.chemical_classification Molecular Structure Macrophages Organic Chemistry Fatty Acids Fatty acid Fibroblasts Enzyme chemistry Molecular Probes Electrophile Click chemistry Molecular Medicine Female HeLa Cells |
| Zdroj: | Bioorganicmedicinal chemistry letters. 18(22) |
| ISSN: | 1464-3405 |
| Popis: | Chemical probes appended with reactive electrophiles afford powerful tools for profiling discrete protein families in living cells. Herein, we have synthesized cell-permeable chemical probes that target fatty acid-associated proteins. These fatty acid-based chemical probes contain acyloxymethylketone or fluorophosphonate functional groups and an alkyne click chemistry tag for visualization of covalently modified proteins by in-gel fluorescence scanning. Our fatty acid-based chemical probe affords new tools to evaluate the activity/expression of lipid-associated proteins that should facilitate their functional characterization and inhibitor discovery. |
| Databáze: | OpenAIRE |
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