Virus-binding activity of fibronectin: Masking of hepatitis A virus
| Autor: | R. Waldherr, Heinrich Liehr, H. P. Seelig, Pott Gerhard, Petra Metzger, Seelig R |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Adult
viruses Radioimmunoassay Virus Feces Affinity chromatography Virology Humans Hepatovirus Viremia Child False Negative Reactions biology fungi Subtilisin Transfusion Reaction virus diseases Hepatitis A biochemical phenomena metabolism and nutrition Molecular biology digestive system diseases Hepatitis a virus Fibronectins Fibronectin biology.protein Antibody Digestion |
| Zdroj: | Journal of Virological Methods. 8:335-347 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/0166-0934(84)90071-5 |
| Popis: | Human plasma fibronectin interacts with viruses. When fibronectin-containing human sera negative for antibodies to hepatitis A virus (HAV) were added to suspensions of HAV, radioimmunological detection of HAV was reduced. This masking effect seemed to depend on the fibronectin concentration of the sera: plasma fibronectin purified by cryoprecipitation and affinity chromatography showed a masking effect on purified HAV which was dependent on the concentrations of fibronectin and HAV. Fibronectin peptides were obtained by subtilisin digestion: the non-collagen-binding regions of the fibronectin molecule were involved in the binding of HAV. We conclude that fibronectin has a virus-binding activity which interferes with radioimmunological methods for virus detection, and may contribute to the frequent transmission of hepatitis viruses by blood products enriched in fibronectin. |
| Databáze: | OpenAIRE |
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