Power assisted liposuction to obtain adipose-derived stem cells: Impact on viability and differentiation to adipocytes in comparison to manual aspiration
| Autor: | Hugo B. Kitzinger, Sonja Wolf, Maximilian Zeyda, Johanna Kober, Otto Riedl, Maike Keck, Thomas M. Stulnig, Alfred Gugerell |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Cell Survival medicine.medical_treatment Gene Expression Adipose tissue Context (language use) 030230 surgery Flow cytometry 03 medical and health sciences 0302 clinical medicine Lipectomy Tissue engineering Adipocytes medicine Humans RNA Messenger Viability assay Cells Cultured Cell Proliferation Glucose Transporter Type 4 medicine.diagnostic_test Adiponectin business.industry Stem Cells Cell Differentiation hemic and immune systems humanities Surgery PPAR gamma 030220 oncology & carcinogenesis Liposuction Tissue and Organ Harvesting Stem cell business |
| Zdroj: | Journal of Plastic, Reconstructive & Aesthetic Surgery; Vol 67 |
| ISSN: | 1748-6815 |
| Popis: | Summary Background Adipose-derived stem cells (ASCs) play a key role in tissue engineering approaches and are probably of major importance in the context of autologous fat transfer. A number of different tools for harvesting ASCs-containing fat tissue have been established. Such devices should be easy to handle, time saving, low priced, safe and provide a high amount of viable ASCs in the aspirate. Power-assisted liposuction (PAL) has not yet been described in the literature as a tool for fat harvesting for lipotranfer. Aim of this study was to investigate ASCs' viability in fat tissue harvested using PAL versus manual aspiration (MA). Methods Fat tissue was obtained from 9 donors undergoing abdominoplasty. Samples were divided into two sections. Out of each section fat was harvested using either PAL or MA. Number of isolated ASCs was defined, proliferation rate was determined and cell viability was assessed by flow cytometry. The ability of isolated ASCs to differentiate into mature adipocytes was analyzed by gene marker expression. Results The number of viable ASCs and the proliferation rates did not significantly differ between PAL and MA but cells harvested using PAL showed significantly higher expression levels of differentiation markers adiponectin, GLUT4 and PPARg. Conclusion Our results show that PAL is a feasible method for harvesting fat tissue containing viable ASCs. Quantity and quality of PAL-harvested ASC is similar or even better, respectively, compared to ASCs harvested by MA. |
| Databáze: | OpenAIRE |
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