Chimeric crRNAs with 19 DNA residues in the guide region show the retained DNA cleavage activity of Cas9 with potential to improve the specificity

Autor:	Jinsu An, Dae-Ro Ahn, Hee Jae Lee, Duhee Bang, Jong Seong Ahn, Cherlhyun Jeong, Jihyun Park, Hak Suk Chung, Seong Jae Kang, Hyo Young Kim, Yongmoon Jeon, Jeong Eun Jang
Rok vydání:	2019
Předmět:	Cellular activity 010402 general chemistry 01 natural sciences Catalysis chemistry.chemical_compound Dna cleavage CRISPR-Associated Protein 9 Materials Chemistry Clustered Regularly Interspaced Short Palindromic Repeats DNA Cleavage Trans-activating crRNA Base Composition 010405 organic chemistry Chemistry Cas9 Metals and Alloys RNA DNA General Chemistry 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Cell biology Ceramics and Composites CRISPR-Cas Systems Target gene RNA Guide Kinetoplastida
Zdroj:	Chemical Communications. 55:3552-3555
ISSN:	1364-548X 1359-7345
DOI:	10.1039/c8cc08468h
Popis:	We demonstrated that 19 out of 20 RNA residues in the guide region of crRNA can be replaced with DNA residues with high GC-contents. The cellular activity of the chimeric crRNAs to disrupt the target gene was comparable to that of the native crRNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3d61a5e0481fe4c4847dfc6f9a01075 https://doi.org/10.1039/c8cc08468h Zobrazit plný text záznamu