Cell kinetics during regeneration in the sponge Halisarca caerulea: how local is the response to tissue damage?
| Autor: | Harm G. van der Geest, Michelle Achlatis, Bert Schutte, Ronald Osinga, Jack P.M. Cleutjens, Jasper M. de Goeij, Brittany E. Alexander |
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| Přispěvatelé: | Aquatic Environmental Ecology (IBED, FNWI), Pathologie, Moleculaire Celbiologie, RS: CARIM - R3 - Vascular biology, RS: GROW - Oncology, RS: GROW - R2 - Basic and Translational Cancer Biology |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
Anatomy and Physiology Cell kinetics population lcsh:Medicine Trade-off Modular integration Aquaculture and Fisheries sublittoral demosponge trade-off coral-reef sponges education.field_of_study Ecology biology integumentary system Choanocyte Aquacultuur en Visserij General Neuroscience General Medicine Cell cycle Immunohistochemistry Cell biology Sponges morphological strategies Collagen General Agricultural and Biological Sciences medicine.medical_specialty Histology Early Regeneration growth Population Marine Biology General Biochemistry Genetics and Molecular Biology medicine Mesohyl Regeneration Choanocyte turnover 14. Life underwater education Cell growth Regeneration (biology) life-history lcsh:R Cell Biology biology.organism_classification cycle checkpoints gene-expression Sponge WIAS chemical defenses |
| Zdroj: | PeerJ, Vol 3, p e820 (2015) PeerJ 3 (2015) PeerJ, 3:e820. PeerJ PeerJ PeerJ, 3 PEERJ, 3:e820. PeerJ Inc. |
| ISSN: | 2167-8359 |
| DOI: | 10.7717/peerj.820 |
| Popis: | Sponges have a remarkable capacity to rapidly regenerate in response to wound infliction. In addition, sponges rapidly renew their filter systems (choanocytes) to maintain a healthy population of cells. This study describes the cell kinetics of choanocytes in the encrusting reef sponge Halisarca caerulea during early regeneration (0-8 h) following experimental wound infliction. Subsequently, we investigated the spatial relationship between regeneration and cell proliferation over a six-day period directly adjacent to the wound, 1 cm, and 3 cm from the wound. Cell proliferation was determined by the incorporation of 5-bromo-2'-deoxyuridine (BrdU). We demonstrate that during early regeneration, the growth fraction of the choanocytes (i.e., the percentage of proliferative cells) adjacent to the wound is reduced (7.0 +/- 2.5%) compared to steady-state, undamaged tissue (46.6 +/- 2.6%), while the length of the cell cycle remained short (5.6 +/- 3.4 h). The percentage of proliferative choanocytes increased over time in all areas and after six days of regeneration choanocyte proliferation rates were comparable to steady-state tissue. Tissue areas farther from the wound had higher rates of choanocyte proliferation than areas closer to the wound, indicating that more resources are demanded from tissue in the immediate vicinity of the wound. There was no difference in the number of proliferative mesohyl cells in regenerative sponges compared to steady-state sponges. Our data suggest that the production of collagen-rich wound tissue is a key process in tissue regeneration for H. caerulea, and helps to rapidly occupy the bare substratum exposed by the wound. Regeneration and choanocyte renewal are competing and negatively correlated life-history traits, both essential to the survival of sponges. The efficient allocation of limited resources to these life-history traits has enabled the ecological success and diversification of sponges. |
| Databáze: | OpenAIRE |
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