Investigating Host Microbiota Relationships Through Functional Metagenomics
| Autor: | Elisabeth Laville, Josette Perrier, Nada Bejar, Marc Maresca, Jeremy Esque, Alexandra S. Tauzin, Emna Bouhajja, Marion Leclerc, Elodie Drula, Bernard Henrissat, Stephane Berdah, Eric Di Pasquale, Patrick Robe, Gabrielle Potocki-Veronese |
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| Přispěvatelé: | Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Institut des Sciences Moléculaires de Marseille (ISM2), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Biodiversité et Biotechnologie Fongiques (BBF), École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Architecture et fonction des macromolécules biologiques (AFMB), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biomécanique Appliquée (LBA UMR T24), Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-Aix Marseille Université (AMU), Institut de neurophysiopathologie (INP), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Givaudan France Arômes SA, French Ministry of Education and Research (Ministere de l'Enseignement Superieur et de la Recherche), INRA's M2E Metaprogramme (the Metascreen project), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Aix Marseille Université (AMU)-Université Gustave Eiffel, European Project: 685474,H2020,H2020-LEIT-BIO-2015-1,METAFLUIDICS(2016), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR) |
| Rok vydání: | 2018 |
| Předmět: |
Microbiology (medical)
Glycan lcsh:QR1-502 microbiote digestif functional metagenomics Sialidase Microbiology lcsh:Microbiology mucus intestinal 03 medical and health sciences human gut microbiota human intestinal mucin 030304 developmental biology Original Research lectin binding 0303 health sciences glycan biology criblage à haut debit 030306 microbiology Microbiology and Parasitology Mucin carbohydrate-active enzymes biology.organism_classification Isolation (microbiology) Microbiologie et Parasitologie bactérie digestive Fosmid Metabolic pathway [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Biochemistry Metagenomics glycane biology.protein Bacteroides |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Frontiers Media, 2019, 10, 12 p. ⟨10.3389/fmicb.2019.01286⟩ Frontiers in Microbiology, 2019, 10, 12 p. ⟨10.3389/fmicb.2019.01286⟩ Frontiers in Microbiology, Vol 10 (2019) Frontiers in Microbiology (10), 12 p.. (2019) |
| ISSN: | 1664-302X |
| Popis: | The human Intestinal mucus is formed by glycoproteins, the O- and N-linked glycans which constitute a crucial source of carbon for commensal gut bacteria, especially when deprived of dietary glycans of plant origin. In recent years, a dozen carbohydrate-active enzymes from cultivated mucin degraders have been characterized. But yet, considering the fact that uncultured species predominate in the human gut microbiota, these biochemical data are far from exhaustive. In this study, we used functional metagenomics to identify new metabolic pathways in uncultured bacteria involved in harvesting mucin glycans. First, we performed a high-throughput screening of a fosmid metagenomic library constructed from the ileum mucosa microbiota using chromogenic substrates. The screening resulted in the isolation of 124 clones producing activities crucial in the degradation of human O- and N-glycans, namely sialidases, beta-D-N-acetyl-glucosaminidase, beta-D-N-acetyl-galactosaminidase, and/or beta-D-mannosidase. Thirteen of these clones were selected based on their diversified functional profiles and were further analyzed on a secondary screening. This step consisted of lectin binding assays to demonstrate the ability of the clones to degrade human intestinal mucus. In total, the structural modification of several mucin motifs, sialylated mucin ones in particular, was evidenced for nine clones. Sequencing their metagenomic loci highlighted complex catabolic pathways involving the complementary functions of glycan sensing, transport, hydrolysis, deacetylation, and deamination, which were sometimes associated with amino acid metabolism machinery. These loci are assigned to several Bacteroides and Feacalibacterium species highly prevalent and abundant in the gut microbiome and explain the metabolic flexibility of gut bacteria feeding both on dietary and human glycans. |
| Databáze: | OpenAIRE |
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