Adaptation of a Potyvirus Chimera Increases Its Virulence in a Compatible Host through Changes in HCPro
| Autor: | Hao Sun, Francisco del Toro, Mongia Makki, Francisco Tenllado, Tomas Canto |
|---|---|
| Přispěvatelé: | Sun, Hao, Del Toro, Francisco, Makki, Mongia, Tenllado, Francisco, Canto, Tomás |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Viral infectious cycle
Viral adaptation Ecology Viral host-range HCPro suppressor of silencing Potyviral non-persistent transmission Plant Science Antiviral silencing suppression viral adaptation potyvirus virulence chimeric viruses antiviral silencing suppression viral infectious cycle potyviral non-persistent transmission viral host-range Potyvirus virulence Ecology Evolution Behavior and Systematics Chimeric viruses |
| Zdroj: | Plants; Volume 11; Issue 17; Pages: 2262 |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants11172262 |
| Popis: | A viral chimera in which the P1-HCPro bi-cistron of a plum pox virus construct (PPV-GFP) was replaced by that of potato virus Y (PVY) spread slowly systemically in Nicotiana benthamiana plants and accumulated to levels that were 5-10% those of parental PPV-GFP. We tested whether consecutive mechanical passages could increase its virulence, and found that after several passages,chimera titers rose and symptoms increased. We sequenced over half the genome of passaged chimera lineages infecting two plants. The regions sequenced were 50NCR-P1-HCPro-P3; Vpg/NIa;GFP-CP, because of being potential sites for mutations/deletions leading to adaptation. We found few substitutions, all non-synonymous: two in one chimera (nt 2053 HCPro, and 5733 Vpg/NIa), and three in the other (2359 HCPro, 5729 Vpg/NIa, 9466 CP). HCPro substitutions 2053 AUU(Ile)!ACU(Thr),and 2359 CUG(Leu)!CGG(Arg) occurred at positions where single nucleotide polymorphisms were observed in NGS libraries of sRNA reads from agroinfiltrated plants (generation 1). Remarkably, position 2053 was the only one in the sequenced protein-encoding genome in which polymorphisms were common to the four libraries, suggesting that selective pressure existed to alter that specific nucleotide, previous to any passage. Mutations 5729 and 5733 in the Vpg by contrast did not correlate with polymorphisms in generation 1 libraries. Reverse genetics showed that substitution 2053 alone increased several-fold viral local accumulation, speed of systemic spread, and systemic titers. This work was funded by a Research Grant from the Spanish Ministry of Innovation and Science (PID2019-109304RB-I00). |
| Databáze: | OpenAIRE |
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