Cell death detection by quantitative three-dimensional single-cell tomography
| Autor: | Jeng-Wei Tjiu, Chien-Chih Lai, Sheng-Lung Huang, Tsung-Hsun Hsieh, Nai-Chia Cheng, Yu-Ta Wang, Ming-Yi Lin, Chia-Kai Chang, Ding-Wei Huang |
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| Rok vydání: | 2012 |
| Předmět: |
In situ
Pathology medicine.medical_specialty Materials science genetic structures Cell ocis:(100.2960) Image analysis Light scattering law.invention Optical coherence tomography Confocal microscopy law medicine ocis:(170.4500) Optical coherence tomography ocis:(170.1870) Dermatology Basal cell carcinoma Image resolution medicine.diagnostic_test medicine.disease Atomic and Molecular Physics and Optics eye diseases Cell Studies medicine.anatomical_structure ocis:(170.1530) Cell analysis Tomography sense organs Biotechnology Biomedical engineering |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Popis: | Ultrahigh-resolution optical coherence tomography (UR-OCT) has been used for the first time to our knowledge to study single-cell basal cell carcinoma (BCC) in vitro. This noninvasive, in situ, label-free technique with deep imaging depth enables three-dimensional analysis of scattering properties of single cells with cellular spatial resolution. From three-dimensional UR-OCT imaging, live and dead BCC cells can be easily identified based on morphological observation. We developed a novel method to automatically extract characteristic parameters of a single cell from data volume, and quantitative comparison and parametric analysis were performed. The results demonstrate the capability of UR-OCT to detect cell death at the cellular level. |
| Databáze: | OpenAIRE |
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